Characterization of the Aedes albopictus (Diptera: Culicidae) holobiome: bacterial composition across land use type and mosquito sex in Malaysia

•Bacteria composition of Aedes albopictus is sourced from the environment and diet.•Land use type and mosquito sex influenced bacteria community.•Wolbachia exhibited co-exclusionary interactions with other resident bacteria.•Wolbachia biocontrol strategy need to consider the density of local Wolbach...

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Bibliographic Details
Published in:Acta tropica 2020-12, Vol.212, p.105683-105683, Article 105683
Main Authors: Lee, J.M., Yek, S.H., Wilson, R.F., Rahman, S.
Format: Article
Language:English
Subjects:
16S rRNA sequencing
Aedes - microbiology
Animals
Bacteria - isolation & purification
Female
Malaysia
Male
Microbial interaction network
Microbial Interactions
Microbiota
Wolbachia
Wolbachia - isolation & purification
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Summary:•Bacteria composition of Aedes albopictus is sourced from the environment and diet.•Land use type and mosquito sex influenced bacteria community.•Wolbachia exhibited co-exclusionary interactions with other resident bacteria.•Wolbachia biocontrol strategy need to consider the density of local Wolbachia strain. Understanding the diversity and dynamics of the microbiota within the mosquito holobiome is of great importance to apprehend how the microbiota modulates various complex processes and interactions. This study examined the bacterial composition of Aedes albopictus across land use type and mosquito sex in the state of Selangor, Malaysia using 16S rRNA sequencing. The bacterial community structure in mosquitoes was found to be influenced by land use type and mosquito sex, with the environment and mosquito diet respectively identified to be the most likely sources of microbes. We found that approximately 70% of the microbiota samples were dominated by Wolbachia and removing Wolbachia from analyses revealed the relatively even composition of the remaining bacterial microbiota. Furthermore, microbial interaction network analysis highlighted the prevalence of co-exclusionary patterns in all networks regardless of land use and mosquito sex, with Wolbachia exhibiting co-exclusionary interactions with other residential bacteria such as Xanthomonas, Xenophilus and Zymobacter. [Display omitted]
ISSN:0001-706X
1873-6254
DOI:10.1016/j.actatropica.2020.105683