Application of dielectrophoresis towards characterization of rare earth elements biosorption by Cupriavidus necator

• Published in: Analytica chimica acta 2020-09, Vol.1129, p.150-157
• Main Authors: Adekanmbi, Ezekiel O., Giduthuri, Anthony T., Carv, Bennett A.C., Counts, Jonathan, Moberly, James G., Srivastava, Soumya K.
• Format: Article
• Language: English
• Subjects: Bacteria; Biomass; Crossover frequency; Cupriavidus necator; Dielectric characterization; Dielectrophoresis; Europium; Rare earth elements
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2020.07.042

This work presents the dielectric characterization of rare earth elements (REEs) biosorption by Cupriavidus necator using dielectrophoretic crossover frequency measurements. Traditional means of characterizing biomass for biosorption is limited and time consuming. In this research we are presenting, for the first time, an electrokinetic method termed as dielectrophoresis (DEP) for the characterization of biosorption (uptake) of rare earth elements (REEs) by gram negative bacteria - Cupriavidus necator. To characterize, a 3mm-diameter point and planar microwell device platform is used to measure the DEP crossover frequency that yields the dielectric properties of the targeted biosorbents. Quantified dielectric properties of native Cupriavidus necator (REE−) and those exposed to rare earth elements (REE+): europium, neodymium, and samarium revealed a substantial change in the surface characteristics of the Cupriavidus necator after exposure to the REE solution. The response of C. necator to changes in REE exposure is substantially different for europium but similar between neodymium and samarium. Statistically both the REE+ and REE− groups dielectric signatures were significantly different proving that the REEs were absorbed by the bacteria. This research will revolutionize and impact the researchers and industrialists in the field of biosorption seeking for economical, greener, and sustainable means to recover REEs. [Display omitted] •Traditional means of characterizing biomass for biosorption is time consuming.•Dielectrophoresis for biosorption by Cupriavidus necator shows promise.•Point and planar microwell to quantify the dielectric properties of the biosorbents.•Quantified dielectric properties of native and REE exposed bacteria revealed changes.•This research will revolutionize and impact the field of biosorption to recover REEs.