Biomimetic cues from poly(lactic--glycolic acid)/hydroxyapatite nano-fibrous scaffolds drive osteogenic commitment in human mesenchymal stem cells in the absence of osteogenic factor supplements

Mimicking the complex hierarchical architecture of the 'osteon', the functional unit of cortical bone, from the bottom-up offers the possibility of generating mature bone tissue in tissue engineered bone substitutes. In this work, a modular 'bottom-up' approach has been developed...

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Bibliographic Details
Published in:Biomaterials science 2020-10, Vol.8 (2), p.5677-5689
Main Authors: Soheilmoghaddam, Mohammad, Padmanabhan, Harish, Cooper-White, Justin J
Format: Article
Language:English
Subjects:
Alizarin
Biomedical materials
Biomimetics
Body fluids
Bone marrow
Cell Differentiation
Cell Proliferation
Cells, Cultured
Cues
Differentiation (biology)
Durapatite
Electrospinning
Glycolic acid
Glycols
Humans
Hydroxyapatite
In vitro methods and tests
Lactic Acid
Mechanical properties
Mesenchymal Stem Cells
Modular engineering
Nanocomposites
Nanofibers
Osteoblasts
Osteogenesis
Polylactic Acid-Polyglycolic Acid Copolymer
Scaffolds
Size distribution
Staining
Stem cells
Substitute bone
Surface roughness
Surgical implants
Tissue Engineering
Tissue Scaffolds
Water drops
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Summary:Mimicking the complex hierarchical architecture of the 'osteon', the functional unit of cortical bone, from the bottom-up offers the possibility of generating mature bone tissue in tissue engineered bone substitutes. In this work, a modular 'bottom-up' approach has been developed to assemble bone niche-mimicking nanocomposite scaffolds composed of aligned electrospun nanofibers of poly(lactic- co -glycolic acid) (PLGA) encapsulating aligned rod-shape nano-sized hydroxyapatite (nHA). By encoding axial orientation of the nHA within these aligned nanocomposite fibers, significant improvements in mechanical properties, surface roughness, hydrophilicity and in vitro simulated body fluid (SBF) mineral deposition were achieved. Moreover, these hierarchical scaffolds induced robust formation of bone hydroxyapatite and osteoblastic maturation of human bone marrow-derived mesenchymal stem cells (hBMSCs) in growth media that was absent of any soluble osteogenic differentiation factors. The results of this investigation confirm that these tailored, aligned nanocomposite fibers, in the absence of media-bone inductive factors, offer the requisite biophysical and biochemical cues to hBMSCs to promote and support their differentiation into mature osteoblast cells and form early bone-like tissue in vitro . Culturing of hBMSCs on aligned electrospun nanocomposite fibers, composed of PLGA modified with rod-shape n-HA.
ISSN:2047-4830
2047-4849
DOI:10.1039/d0bm00946f