Enzyme immobilization on glass fiber membrane for detection of halogenated compounds

• Published in: Analytical biochemistry 2020-11, Vol.609, p.113971-113971, Article 113971
• Main Authors: Gul, Ijaz, Wang, Qian, Jiang, Qifa, Fang, Ruiqin, Tang, Lixia
• Format: Article
• Language: English
• Subjects: Agrobacterium tumefaciens - enzymology; alpha-Chlorohydrin - analogs & derivatives; alpha-Chlorohydrin - analysis; Colorimetry - methods; Enzymatic detection; Enzyme Stability; Enzymes, Immobilized - chemistry; Enzymes, Immobilized - metabolism; Fresh Water - analysis; Glass - chemistry; Glass fiber membrane; Halogenated compounds; Halohydrin dehalogenase (HheC); Hydrogen-Ion Concentration; Hydrolases - chemistry; Hydrolases - genetics; Hydrolases - metabolism; Immobilization; Limit of Detection; Propanols - analysis; Recombinant Proteins - biosynthesis; Recombinant Proteins - chemistry; Recombinant Proteins - isolation & purification; Temperature; Water Pollutants, Chemical - analysis
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/j.ab.2020.113971

Enzyme immobilization using inorganic membranes has enticed increased attention as they not only improve enzyme stability, but also furnish user-friendly biodevices that can be tailored to different applications. Herein, we explored the suitability of the glass fiber membrane for enzyme immobilization and its application for halocarbon detection. For this, halohydrin dehalogenase (HheC) and bovine serum albumin were crosslinked and immobilized on a glass fiber membrane without membrane functionalization. Immobilized HheC exhibited higher storage stability than its free counterpart over 60 days at 4 °C (67% immobilized vs. 8.1% free) and 30 °C (77% immobilized vs. 57% free). Similarly, the thermal endurance of the immobilized HheC was significantly improved. The practical utility of the membrane-immobilized enzyme was demonstrated by colorimetric detection of 1,3-dichloro-2-propanol (1,3-DCP) and 2,3-dibromo-1-propanol (2,3-DBP) as model analytes. Under optimized conditions, the detection limits of 0.06 mM and 0.09 mM were achieved for 1,3-DCP and 2,3-DBP, respectively. The satisfactory recoveries were observed with spiked river and lake water samples, which demonstrate the application potential of immobilized HheC for screening contaminants in water samples. Our results revealed that the proposed frugal and facile approach could be useful for enzyme stabilization, and mitigation of halocarbon pollution. [Display omitted] •Immobilized HheC exhibited higher thermal endurance and storage stability than free HheC.•Glass fiber membrane was found to be a suitable support matrix.•Halocarbons were detected in standard solution and environmental samples.