Phylogeny of hospital acquired MRSA, and its comparative phenotypic clinico-epidemiology with vancomycin resistant S. aureus (VRSA)

• Published in: Microbial pathogenesis 2020-12, Vol.149, p.104537-104537, Article 104537
• Main Authors: Rehman, Tayyab ur, Aslam, Rizwan, Aqib, Amjad Islam, Mohsin, Mashkoor, Manzoor, Asad, Shoaib, Muhammad, Naseer, Muhammad Aamir, Hasan, Ali, Sattar, Huma, Fakhar-e-Alam Kulyar, Muhammad, Muzammil, Iqra, Yao, Wangyuan
• Format: Article
• Language: English
• Subjects: Antibiotic susceptibility assay; Epidemiology; HA-MRSA; Phylogeny; Risk factors; VRSA
• ISSN: 0882-4010; 1096-1208
• DOI: 10.1016/j.micpath.2020.104537

Staphylococcus aureus is emerging as complicated pathogen because of its wide-ranging origin, multiple variants, and compromised antibiotic susceptibilities. Current study was planned to find lineage of hospital acquired methicillin resistant Staphylococcus aureus (HA-MRSA), and its comparative phenotypic clinico-epidemiology with vancomycin resistant S. aureus (VRSA). A total of (n = 200) samples were aseptically collected from wound, nose, and cerebrospinal fluid of patients from metropolitan and rural background hospitals along with on spot filling in of questionnaire. Phylogenetic analysis of HA-MRSA was identified by targeting mecA gene in S. aureus. At optimal tree branch length of 1.91 and evolutionary distance 0.1, high level sequence similarity (97%–99%) was observed with different strains of S. aureus isolated from both human and animal. Non-descriptive statistics at 5% probability found 61% S. aureus, while 43.44% of them were HA-MRSA, 92.62% VRSA, and 42.62% were both MRSA and VRSA. Among assumed risk factors, use of antibiotics, venous catheterization, chronic disease, pre-hospital visits, and ICU admitted patients showed significant association (p