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Histological and cellular evaluation of anterior cruciate ligament
We hypothesized that the torn anterior cruciate ligament (ACL) demonstrates a great healing response after initial trauma and has competent cells leading to the healing but differs in its response based on the type of tear and duration of injury. This study aimed to evaluate the histological and cel...
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| Published in: | The knee 2020-10, Vol.27 (5), p.1510-1518 |
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| Main Authors: | , , , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c384t-7a05af6c53fc8853d4dfbb38b21f42b602d592bd9997735d12cbfbbc83855c803 |
| container_end_page | 1518 |
| container_issue | 5 |
| container_start_page | 1510 |
| container_title | The knee |
| container_volume | 27 |
| creator | Butt, Umer Khan, Zainab Aqeel Zahir, Naila Khan, Zeeshan Vuletic, Filip Shah, Imran Shah, Junaid Ali Siddiqui, Athar Muniruddin Hudetz, Damir |
| description | We hypothesized that the torn anterior cruciate ligament (ACL) demonstrates a great healing response after initial trauma and has competent cells leading to the healing but differs in its response based on the type of tear and duration of injury. This study aimed to evaluate the histological and cellular responses to the injured ACL.
Fifty-two tissue samples from the ACL were harvested from patients undergoing arthroscopy. Detailed histological and cellular examinations were performed for ligament angiogenesis, fibrocytes, and synovial tissue infiltration. We compared the cellular response to injury in partially and completely ruptured ACLs. The duration of ACL injury and its response to cellular characteristics were also examined. Immunohistochemical studies using cluster of differentiation 34 (CD34) staining was used to evaluate endothelial cells and fibrocytes.
We found a significantly higher density of synovial and ligament angiogenesis and fibrocytes at the torn end of ACL (Mann–Whitney, P |
| doi_str_mv | 10.1016/j.knee.2020.08.002 |
| format | article |
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Fifty-two tissue samples from the ACL were harvested from patients undergoing arthroscopy. Detailed histological and cellular examinations were performed for ligament angiogenesis, fibrocytes, and synovial tissue infiltration. We compared the cellular response to injury in partially and completely ruptured ACLs. The duration of ACL injury and its response to cellular characteristics were also examined. Immunohistochemical studies using cluster of differentiation 34 (CD34) staining was used to evaluate endothelial cells and fibrocytes.
We found a significantly higher density of synovial and ligament angiogenesis and fibrocytes at the torn end of ACL (Mann–Whitney, P < 0.050). Numerous fibrocytes were identified in complete ACL tears versus partial tears (Mann–Whitney = 0.020). Increased cellular proliferation was identified at the ruptured end of ACL remnant (Kruskal–Wallis, P < 0.050). The cellular proliferation of ruptured ACL decreased after 12 months.
Based on our findings of the time-dependent decrease in the cellular response at the torn ends of the ACL, we recommend early intervention, preservation of the ACL remnant, and primary ACL repair or augmented reconstruction.</description><identifier>ISSN: 0968-0160</identifier><identifier>EISSN: 1873-5800</identifier><identifier>DOI: 10.1016/j.knee.2020.08.002</identifier><identifier>PMID: 33010768</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adolescent ; Adult ; Angiogenesis ; Anterior cruciate ligament ; Anterior cruciate ligament (ACL) ; Anterior Cruciate Ligament - blood supply ; Anterior Cruciate Ligament - pathology ; Anterior Cruciate Ligament Injuries - pathology ; Arthroscopy ; Biopsy ; Cartilage ; CD34 antigen ; Cell Proliferation ; Endothelial cells ; Female ; Fibroblasts - pathology ; Fibrocytes ; Histology ; Humans ; Injuries ; Knee ; Ligaments ; Male ; Middle Aged ; Neovascularization, Physiologic ; Prospective Studies ; Stump preserving reconstruction ; Trauma ; Variance analysis ; Young Adult</subject><ispartof>The knee, 2020-10, Vol.27 (5), p.1510-1518</ispartof><rights>2020 Elsevier B.V.</rights><rights>Copyright © 2020 Elsevier B.V. All rights reserved.</rights><rights>2020. Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-7a05af6c53fc8853d4dfbb38b21f42b602d592bd9997735d12cbfbbc83855c803</citedby><cites>FETCH-LOGICAL-c384t-7a05af6c53fc8853d4dfbb38b21f42b602d592bd9997735d12cbfbbc83855c803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33010768$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Butt, Umer</creatorcontrib><creatorcontrib>Khan, Zainab Aqeel</creatorcontrib><creatorcontrib>Zahir, Naila</creatorcontrib><creatorcontrib>Khan, Zeeshan</creatorcontrib><creatorcontrib>Vuletic, Filip</creatorcontrib><creatorcontrib>Shah, Imran</creatorcontrib><creatorcontrib>Shah, Junaid Ali</creatorcontrib><creatorcontrib>Siddiqui, Athar Muniruddin</creatorcontrib><creatorcontrib>Hudetz, Damir</creatorcontrib><title>Histological and cellular evaluation of anterior cruciate ligament</title><title>The knee</title><addtitle>Knee</addtitle><description>We hypothesized that the torn anterior cruciate ligament (ACL) demonstrates a great healing response after initial trauma and has competent cells leading to the healing but differs in its response based on the type of tear and duration of injury. This study aimed to evaluate the histological and cellular responses to the injured ACL.
Fifty-two tissue samples from the ACL were harvested from patients undergoing arthroscopy. Detailed histological and cellular examinations were performed for ligament angiogenesis, fibrocytes, and synovial tissue infiltration. We compared the cellular response to injury in partially and completely ruptured ACLs. The duration of ACL injury and its response to cellular characteristics were also examined. Immunohistochemical studies using cluster of differentiation 34 (CD34) staining was used to evaluate endothelial cells and fibrocytes.
We found a significantly higher density of synovial and ligament angiogenesis and fibrocytes at the torn end of ACL (Mann–Whitney, P < 0.050). Numerous fibrocytes were identified in complete ACL tears versus partial tears (Mann–Whitney = 0.020). Increased cellular proliferation was identified at the ruptured end of ACL remnant (Kruskal–Wallis, P < 0.050). The cellular proliferation of ruptured ACL decreased after 12 months.
Based on our findings of the time-dependent decrease in the cellular response at the torn ends of the ACL, we recommend early intervention, preservation of the ACL remnant, and primary ACL repair or augmented reconstruction.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Angiogenesis</subject><subject>Anterior cruciate ligament</subject><subject>Anterior cruciate ligament (ACL)</subject><subject>Anterior Cruciate Ligament - blood supply</subject><subject>Anterior Cruciate Ligament - pathology</subject><subject>Anterior Cruciate Ligament Injuries - pathology</subject><subject>Arthroscopy</subject><subject>Biopsy</subject><subject>Cartilage</subject><subject>CD34 antigen</subject><subject>Cell Proliferation</subject><subject>Endothelial cells</subject><subject>Female</subject><subject>Fibroblasts - pathology</subject><subject>Fibrocytes</subject><subject>Histology</subject><subject>Humans</subject><subject>Injuries</subject><subject>Knee</subject><subject>Ligaments</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Neovascularization, Physiologic</subject><subject>Prospective Studies</subject><subject>Stump preserving reconstruction</subject><subject>Trauma</subject><subject>Variance analysis</subject><subject>Young Adult</subject><issn>0968-0160</issn><issn>1873-5800</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LxDAURYMoOn78ARdScOOm9TVp2hTc6KCOILjRdUiTV8nYacakHfDfmzKjCxeu3uKde7kcQs5zyHLIy-tl9tEjZhQoZCAyALpHZrmoWMoFwD6ZQV2KNJJwRI5DWAJAWRf8kBwxBjlUpZiRu4UNg-vcu9WqS1RvEo1dN3bKJ7hR3agG6_rEtfE1oLfOJ9qP2qoBk86-qxX2wyk5aFUX8Gx3T8jbw_3rfJE-vzw-zW-fU81EMaSVAq7aUnPWaiE4M4Vpm4aJhuZtQZsSqOE1bUxd11XFuMmpbiKgBROcawHshFxte9fefY4YBrmyYVqrenRjkLQoRAEVL-uIXv5Bl270fVwXKS5qzgRMFN1S2rsQPLZy7e1K-S-Zg5wMy6WcDMvJsAQho-EYuthVj80KzW_kR2kEbrYARhcbi14GbbHXaKxHPUjj7H_939o3i9I</recordid><startdate>202010</startdate><enddate>202010</enddate><creator>Butt, Umer</creator><creator>Khan, Zainab Aqeel</creator><creator>Zahir, Naila</creator><creator>Khan, Zeeshan</creator><creator>Vuletic, Filip</creator><creator>Shah, Imran</creator><creator>Shah, Junaid Ali</creator><creator>Siddiqui, Athar Muniruddin</creator><creator>Hudetz, Damir</creator><general>Elsevier B.V</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>202010</creationdate><title>Histological and cellular evaluation of anterior cruciate ligament</title><author>Butt, Umer ; Khan, Zainab Aqeel ; Zahir, Naila ; Khan, Zeeshan ; Vuletic, Filip ; Shah, Imran ; Shah, Junaid Ali ; Siddiqui, Athar Muniruddin ; Hudetz, Damir</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-7a05af6c53fc8853d4dfbb38b21f42b602d592bd9997735d12cbfbbc83855c803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Angiogenesis</topic><topic>Anterior cruciate ligament</topic><topic>Anterior cruciate ligament (ACL)</topic><topic>Anterior Cruciate Ligament - blood supply</topic><topic>Anterior Cruciate Ligament - pathology</topic><topic>Anterior Cruciate Ligament Injuries - pathology</topic><topic>Arthroscopy</topic><topic>Biopsy</topic><topic>Cartilage</topic><topic>CD34 antigen</topic><topic>Cell Proliferation</topic><topic>Endothelial cells</topic><topic>Female</topic><topic>Fibroblasts - pathology</topic><topic>Fibrocytes</topic><topic>Histology</topic><topic>Humans</topic><topic>Injuries</topic><topic>Knee</topic><topic>Ligaments</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Neovascularization, Physiologic</topic><topic>Prospective Studies</topic><topic>Stump preserving reconstruction</topic><topic>Trauma</topic><topic>Variance analysis</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Butt, Umer</creatorcontrib><creatorcontrib>Khan, Zainab Aqeel</creatorcontrib><creatorcontrib>Zahir, Naila</creatorcontrib><creatorcontrib>Khan, Zeeshan</creatorcontrib><creatorcontrib>Vuletic, Filip</creatorcontrib><creatorcontrib>Shah, Imran</creatorcontrib><creatorcontrib>Shah, Junaid Ali</creatorcontrib><creatorcontrib>Siddiqui, Athar Muniruddin</creatorcontrib><creatorcontrib>Hudetz, Damir</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>The knee</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Butt, Umer</au><au>Khan, Zainab Aqeel</au><au>Zahir, Naila</au><au>Khan, Zeeshan</au><au>Vuletic, Filip</au><au>Shah, Imran</au><au>Shah, Junaid Ali</au><au>Siddiqui, Athar Muniruddin</au><au>Hudetz, Damir</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Histological and cellular evaluation of anterior cruciate ligament</atitle><jtitle>The knee</jtitle><addtitle>Knee</addtitle><date>2020-10</date><risdate>2020</risdate><volume>27</volume><issue>5</issue><spage>1510</spage><epage>1518</epage><pages>1510-1518</pages><issn>0968-0160</issn><eissn>1873-5800</eissn><abstract>We hypothesized that the torn anterior cruciate ligament (ACL) demonstrates a great healing response after initial trauma and has competent cells leading to the healing but differs in its response based on the type of tear and duration of injury. This study aimed to evaluate the histological and cellular responses to the injured ACL.
Fifty-two tissue samples from the ACL were harvested from patients undergoing arthroscopy. Detailed histological and cellular examinations were performed for ligament angiogenesis, fibrocytes, and synovial tissue infiltration. We compared the cellular response to injury in partially and completely ruptured ACLs. The duration of ACL injury and its response to cellular characteristics were also examined. Immunohistochemical studies using cluster of differentiation 34 (CD34) staining was used to evaluate endothelial cells and fibrocytes.
We found a significantly higher density of synovial and ligament angiogenesis and fibrocytes at the torn end of ACL (Mann–Whitney, P < 0.050). Numerous fibrocytes were identified in complete ACL tears versus partial tears (Mann–Whitney = 0.020). Increased cellular proliferation was identified at the ruptured end of ACL remnant (Kruskal–Wallis, P < 0.050). The cellular proliferation of ruptured ACL decreased after 12 months.
Based on our findings of the time-dependent decrease in the cellular response at the torn ends of the ACL, we recommend early intervention, preservation of the ACL remnant, and primary ACL repair or augmented reconstruction.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>33010768</pmid><doi>10.1016/j.knee.2020.08.002</doi><tpages>9</tpages></addata></record> |
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| ispartof | The knee, 2020-10, Vol.27 (5), p.1510-1518 |
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| source | ScienceDirect Journals |
| subjects | Adolescent Adult Angiogenesis Anterior cruciate ligament Anterior cruciate ligament (ACL) Anterior Cruciate Ligament - blood supply Anterior Cruciate Ligament - pathology Anterior Cruciate Ligament Injuries - pathology Arthroscopy Biopsy Cartilage CD34 antigen Cell Proliferation Endothelial cells Female Fibroblasts - pathology Fibrocytes Histology Humans Injuries Knee Ligaments Male Middle Aged Neovascularization, Physiologic Prospective Studies Stump preserving reconstruction Trauma Variance analysis Young Adult |
| title | Histological and cellular evaluation of anterior cruciate ligament |
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