Parthenogenetic activation of buffalo ( Bubalus bubalis ) oocytes: comparison of different activation reagents and different media on their developmental competence and quantitative expression of developmentally regulated genes

This study was carried out to compare the efficacy of different methods to activate buffalo A + B and C + D quality oocytes parthenogenetically and to study the in vitro developmental competence of oocytes and expression of some important genes at the different developmental stages of parthenotes. T...

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Bibliographic Details
Published in:Zygote (Cambridge) 2021-02, Vol.29 (1), p.49-58
Main Authors: Singh, K P, Mohapatra, S K, Kaushik, R, Singh, M K, Palta, P, Singla, S K, Manik, R S, Chauhan, M S
Format: Article
Language:English
Subjects:
Abattoirs
Apoptosis
Blastocysts
Buffalo
Cell culture
Chemicals
Developmental stages
Embryogenesis
Embryonic growth stage
Embryos
Ethanol
Fertilization
Gametocytes
Gene expression
Genes
Genomes
Humidity
Oocytes
Ovaries
Polyvinyl alcohol
Reagents
Sperm
Trophectoderm
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Summary:This study was carried out to compare the efficacy of different methods to activate buffalo A + B and C + D quality oocytes parthenogenetically and to study the in vitro developmental competence of oocytes and expression of some important genes at the different developmental stages of parthenotes. The percentage of A + B oocytes (62.16 ± 5.06%, range 53.8-71.3%) was significantly higher (P < 0.001) compared with that of C + D oocytes (37.8 ± 5.00%, range 28.6-46.1%) retrieved from slaughterhouse buffalo ovaries. Among all combinations, ethanol activation followed by culture in research vitro cleave medium gave the highest cleavage and blastocyst yields for both A + B and C + D grade oocytes. Total cell numbers, inner cell mass/trophectoderm ratio and apoptotic index of A + B group blastocysts were significantly different (P < 0.05) from their C + D counterpart. To determine the status of expression patterns of developmentally regulated genes, the expression of cumulus-oocyte complexes, fertilization, developmental competence and apoptotic-related genes were also studied in parthenogenetically produced buffalo embryos at different stages, and indicated that the differential expression patterns of the above genes had a role in early embryonic development.
ISSN:0967-1994
1469-8730
DOI:10.1017/S0967199420000519