Temporal and Spatial Patterns of Zinc and Iron Accumulation during Barley (Hordeum vulgare L.) Grain Development

Breeding and engineering of biofortified crops will benefit from a better understanding of bottlenecks controlling micronutrient loading within the seeds. However, few studies have addressed the changes in micronutrient concentrations, localization, and speciation occurring over time. Therefore, we...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2020-11, Vol.68 (44), p.12229-12240
Main Authors: Detterbeck, Amelie, Pongrac, Paula, Persson, Daniel P, Vogel-Mikuš, Katarina, Kelemen, Mitja, Vavpetič, Primož, Pelicon, Primož, Arčon, Iztok, Husted, Søren, Kofod Schjoerring, Jan, Clemens, Stephan
Format: Article
Language:English
Subjects:
Agricultural and Environmental Chemistry
Edible Grain - chemistry
Edible Grain - growth & development
Edible Grain - metabolism
Endosperm - chemistry
Endosperm - metabolism
Hordeum - chemistry
Hordeum - growth & development
Hordeum - metabolism
Iron - analysis
Iron - metabolism
Micronutrients - analysis
Micronutrients - metabolism
Seeds - chemistry
Seeds - growth & development
Seeds - metabolism
Zinc - analysis
Zinc - metabolism
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Summary:Breeding and engineering of biofortified crops will benefit from a better understanding of bottlenecks controlling micronutrient loading within the seeds. However, few studies have addressed the changes in micronutrient concentrations, localization, and speciation occurring over time. Therefore, we studied spatial patterns of zinc and iron accumulation during grain development in two barley lines with contrasting grain zinc concentrations. Microparticle-induced-X-ray emission and laser ablation-inductively coupled plasma mass spectrometry were used to determine tissue-specific accumulation of zinc, iron, phosphorus, and sulfur. Differences in zinc accumulation between the lines were most evident in the endosperm and aleurone. A gradual decrease in zinc concentrations from the aleurone to the underlying endosperm was observed, while iron and phosphorus concentrations decreased sharply. Iron co-localized with phosphorus in the aleurone, whereas zinc co-localized with sulfur in the sub-aleurone. We hypothesize that differences in grain zinc are largely explained by the endosperm storage capacity. Engineering attempts should be targeted accordingly.
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.0c04833