Smoking and Physical Activity Significantly Influence Stromal Vascular Fraction Cell Yield and Viability

• Published in: Aesthetic plastic surgery 2021-02, Vol.45 (1), p.315-321
• Main Authors: Andjelkov, Katarina, Conde-Green, Alexandra, Mosahebi, Afshin
• Format: Article
• Language: English
• Subjects: Adipose Tissue; Adult; Age; Body mass index; Exercise; Female; Gender; Humans; Lipectomy; Male; Medicine; Medicine & Public Health; Middle Aged; Original Article; Otorhinolaryngology; Patients; Plastic Surgery; Regression analysis; Smoking; Smoking - adverse effects; Stromal Cells; Tissue engineering
• ISSN: 0364-216X; 1432-5241
• DOI: 10.1007/s00266-020-02008-2

Background Subcutaneous tissue is an abundant source of adipose-derived regenerative cells. It is readily available and easy to extract by means of liposuction, making it one of the most popular sources for tissue engineering and regenerative medical applications. Methods The stromal vascular fraction (SVF) cell yield and viability of the lipoaspirate obtained from 43 patients undergoing elective liposuction were examined in correlation with their age, gender, body mass index, smoking status, and physical activity. The lipoaspirate was processed with the Celution ® 800/CRS system to isolate the SVF and a few drops of the obtained pellet were used for cell counting with NecleoCounter ® NC-100 TM . Results Twenty-eight (65.1%) were men and 15 (34.9%) were women with an average age of 40.7 ± 10.4 years (women) and 38.9 ± 11.8 years (men). Viable SVF cells/ g fat was significantly correlated with smoking level (negative correlation, ρ =  − 0.312, P