Large pools and fluxes of carbon, calcium and phosphorus in dense charophyte stands in ponds

• Published in: The Science of the total environment 2021-04, Vol.765, p.142792-142792, Article 142792
• Main Authors: Sand-Jensen, Kaj, Martinsen, Kenneth Thorø, Jakobsen, Anders Lund, Sø, Jonas Stage, Madsen-Østerbye, Mikkel, Kjær, Johan Emil, Kristensen, Emil, Kragh, Theis
• Format: Article
• Language: English
• Subjects: Calcification; Calcium; Carbon; Carbon budgets; Charophyceae; Charophytes; Geologic Sediments; Lakes; Macrophytes; Phosphorus; Phosphorus sink; Ponds
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2020.142792

Bicarbonate and calcium set bounds on photosynthesis and degradation processes in calcareous freshwaters. Charophytic algae use bicarbonate in photosynthesis, and direct variable proportions to assimilate organic carbon and to precipitate calcium carbonate on their surfaces. To evaluate pools of organic carbon (Corg), carbonate carbon (Ccarbonate), and phosphorus (P) in dense charophyte vegetation, we studied apical and basal tissue and carbonate surface precipitates, as well as underlying sediments in ten calcareous ponds. We also quantified the release of calcium, bicarbonate and phosphate from charophyte shoots in dark experiments. We found that the Corg:Ccarbonate quotient in charophyte stands averaged 1.19 during spring and summer. The Corg:Ccarbonate quotient in the sediments formed by dead charophytes averaged 0.97 in accordance with some respiratory CO2 release without carbonate dissolution to bicarbonate. The molar quotient of carbon to calcium was close to 2.0 in sediment and pond water. In dark incubations, shoots subjected to calcium carbonate dissolution released bicarbonate and calcium with a molar quotient of 2:1; lowered pH (7.0–8.0) increased the release. Thus, the carbonate surface crust on living charophytes was not inert, as hitherto anticipated. Phosphate dark release occurred from basal shoots only, was unrelated to pH, and may have derived from organic decomposition, rather than from carbonate dissolution. Extensive phosphorus pools were associated with the charophyte stands (200–600 mg m−2) and had about 2/3 incorporated in alga tissue and 1/3 in carbonate crust. Overall, the biogeochemistry of carbon, calcium and phosphorus are closely linked in calcareous charophyte ponds. Carbonate dissolution from charophyte crusts at night and continuously from sediment might balance extensive carbonate precipitation during daytime photosynthesis. The substantial P-pool in charophyte stands may not derive from P-deprived water, but from P-rich sediment. Charophyte photosynthesis may still contribute to nutrient-poor conditions by forming carbonate-rich sediment of high P-binding capacity. [Display omitted] •Charophytic algae form thick CaCO3 surface crusts in calcareous fresh waters.•In ten ponds, the Corganic:Ccarbonate quotient averaged 1.19 in charophyte stands.•Carbonate dissolution was from sediments and from charophyte crusts at night.•Extensive P-pools in charophyte stands had 2/3 in tissue and 1/3 in surface crust.•Large P-pools in char