Detection and structural characterization of the metabolites of dihydroresveratrol in rats by liquid chromatography coupled to high‐resolution tandem mass spectrometry

Rationale Dihydroresveratrol has been demonstrated to possess a wide spectrum of bioactivities, such as anti‐oxidant and anti‐inflammatory effects. The aim of the present study was to investigate the metabolic profiles of dihydroresveratrol in rats. Methods The in vitro metabolism was elucidated by...

Full description

Saved in:
Bibliographic Details
Published in:Rapid communications in mass spectrometry 2021-02, Vol.35 (4), p.e8991-n/a
Main Authors: Ji, Qiang‐Guo, Ma, Ming‐Hua, Hu, Xue‐Mei, Zhang, Yi‐Jun, Xu, Xiao‐Hong, Nian, Hua
Format: Article
Language:English
Subjects:
Chromatography
Conjugation
Dehydrogenation
Glutathione
Hydroxylation
In vitro methods and tests
Ions
Liquid chromatography
Mass spectrometry
Metabolism
Metabolites
Methylation
Oxidizing agents
Scientific imaging
Spectroscopy
Structural analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Rationale Dihydroresveratrol has been demonstrated to possess a wide spectrum of bioactivities, such as anti‐oxidant and anti‐inflammatory effects. The aim of the present study was to investigate the metabolic profiles of dihydroresveratrol in rats. Methods The in vitro metabolism was elucidated by incubating dihydroresveratrol with rat hepatocytes for 2 h at 37°C. For in vivo metabolism, dihydroresveratrol was orally administered to rats at a single dose of 50 mg/kg and the resulting biliary and urinary samples were collected. All the samples were analyzed by liquid chromatography combined with electrospray ionization high‐resolution mass spectrometry. The structures of the metabolites were proposed based on their accurate masses and their MS/MS product ions. Results A total of 16 metabolites including three phase I metabolites and 13 phase II metabolites were detected and structurally proposed. Among these metabolites, M6 and M14 were unambiguously identified as 3′‐hydroxylresveratrol and resveratrol, respectively, using reference standards. Dihydroresveratrol was mainly metabolized into resveratrol (M14) and a glucuronide conjugate (M12), which were excreted into urine and bile as the major metabolites. Conclusions The metabolic pathways of dihydroresveratrol involved hydroxylation, dehydrogenation, glucuronidation, glutathione (GSH) conjugation and methylation. The present study provided useful information with regard to the metabolic profiles of dihydroresveratrol in rats.
ISSN:0951-4198
1097-0231
DOI:10.1002/rcm.8991