Lactobacillus bulgaricus and Lactobacillus plantarum improve diabetic wound healing through modulating inflammatory factors

• Published in: Biotechnology and applied biochemistry 2021-12, Vol.68 (6), p.1421-1431, Article bab.2064
• Main Authors: Mohtashami, Mahnaz, Mohamadi, Mahsa, Azimi‐Nezhad, Mohsen, Saeidi, Jafar, Nia, Fatemeh Forooghi, Ghasemi, Ahmad
• Format: Article
• Language: English
• Subjects: Animals; Bacteria; Bromodeoxyuridine; Cell Survival - drug effects; Cells, Cultured; Cytokines; Diabetes; Diabetes mellitus; Diabetes Mellitus - drug therapy; Diabetes Mellitus - metabolism; diabetic wound; Fibroblasts; Gene expression; Humans; Inflammation; Inflammation - drug therapy; Inflammation - metabolism; Interleukin-10; Interleukin-1beta - metabolism; Lactobacilli; Lactobacillus bulgaricus; Lactobacillus delbrueckii - chemistry; Lactobacillus plantarum; Lactobacillus plantarum - chemistry; Leukocyte migration; Macrophages; Mice; probiotic; Probiotics; Probiotics - pharmacology; Rats; Rats, Wistar; Strains (organisms); Transforming Growth Factor beta - metabolism; Tumor Necrosis Factor-alpha - metabolism; Wound healing; Wound Healing - drug effects
• ISSN: 0885-4513; 1470-8744
• DOI: 10.1002/bab.2064

Probiotics are nonpathogenic bacterial strains that exert beneficial effects on the host. Previous studies have shown that topical use of some strains of probiotic bacteria have good effects on the healing of cutaneous wounds. In the current study, the wound healing potentials of bacterial probiotics on diabetic cutaneous wounds were evaluated. The effects of probiotics on migration, the viability of fibroblasts, and macrophage proliferation were measured through using wound healing assay, methylthiazol tetrazolium assay, and bromodeoxyuridine, respectively. In this regard, in vivo diabetic wound healing experiments in Wistar rats following treatment with nontoxic concentrations of Lactobacillus bulgaricus and Lactobacillus plantarum were conducted. The histopathological and gene expression analyses were performed following removal of wound sites 3, 7, and 14 days postwounding. Results showed that treatment with probiotics accelerated the healing process of diabetic wounds and modulated the inflammatory cells in wound sites during a 14‐day period postwounding. The altered mRNA levels of inflammatory cytokines were observed in wound sites following treatment with probiotics. The findings of the current study reveal that L. bulgaricus and L. plantarum could improve the healing of diabetic wounds via regulation of inflammation. Treatment with L. bulgaricus and L. plantarum accelerated the wound healing process in the diabetic Wistar rats.