Porcine carotid arteries decellularized with a suitable concentration combination of Triton X-100 and sodium dodecyl sulfate for tissue engineering vascular grafts

Tissue engineering vascular grafts (TEVGs) constructed by decellularized arteries have the potential to replace autologous blood vessels in bypass surgery for patients with cardiovascular disease. There are various methods of decellularization without a standard protocol. Detergents approaches are s...

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Bibliographic Details
Published in:Cell and tissue banking 2021-06, Vol.22 (2), p.277-286
Main Authors: Cai, Zhiwen, Gu, Yongquan, Xiao, Yonghao, Wang, Cong, Wang, Zhonggao
Format: Article
Language:English
Subjects:
Autografts
Biomedical and Life Sciences
Biomedicine
Blood vessels
Cardiovascular diseases
Carotid arteries
Carotid artery
Cell Biology
Detergents
Extracellular matrix
Full Length Paper
Life Sciences
Mechanical properties
Sodium lauryl sulfate
Surgery
Tissue engineering
Transplant Surgery
Veins & arteries
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Summary:Tissue engineering vascular grafts (TEVGs) constructed by decellularized arteries have the potential to replace autologous blood vessels in bypass surgery for patients with cardiovascular disease. There are various methods of decellularization without a standard protocol. Detergents approaches are simple, and easy control of experimental conditions. Non-ionic detergent Triton X-100 and ionic detergent sodium dodecyl sulfate (SDS) are the most commonly used detergents. In this study, we used Triton X-100 and SDS with different concentrations to decellularize porcine carotid arteries. After that, we investigated the acellular effect and mechanical properties of decellularized arteries to find a promising concentration combination for decellularization. Results showed that any detergents’ combination would damage the inherent structure of extracellular matrix, and the destruction increased with the increase of detergents’ concentration. We concluded that the decellularization approach of 0.5% Triton X-100 for 24 h combined with 0.25% SDS for 72 h could help to obtain decellularized arteries with minimum destruction. This protocol may be able to prepare a clinically suitable vascular scaffold for TEVGs.
ISSN:1389-9333
1573-6814
DOI:10.1007/s10561-020-09876-7