Neuroinflammation induces anxiety- and depressive-like behavior by modulating neuronal plasticity in the basolateral amygdala

• Published in: Brain, behavior, and immunity behavior, and immunity, 2021-01, Vol.91, p.505-518
• Main Authors: Zheng, Zhi-Heng, Tu, Jiang-Long, Li, Xiao-Han, Hua, Qing, Liu, Wei-Zhu, Liu, Yu, Pan, Bing-Xing, Hu, Ping, Zhang, Wen-Hua
• Format: Article
• Language: English
• Subjects: Amygdala; Anxiety; Depression; Neural plasticity; Neuroinflammation
• ISSN: 0889-1591; 1090-2139
• DOI: 10.1016/j.bbi.2020.11.007

•Mice treated with LPS induces microglial activation and neuroinflammation in basolateral amygdala.•LPS-treatment increases excitatory, but not inhibitory, neurotransmission onto BLA PNs through a presynaptic mechanism.•LPS treatment increases the intrinsic neuronal excitability of BLA PNs.•Fluoxetine prevents LPS-induced activation of microglial and dysregulation of neuronal plasticity of BLA PNs. Increasing evidence indicates that excessive inflammatory responses play a crucial role in the pathophysiology of psychiatric diseases, including depression and anxiety disorders. The dysfunctional neural plasticity in amygdala has long been proposed as the vital cause for the progression of psychiatric disorders. However, the effect of neuroinflammation on the functional changes of the amygdala remains largely unknown. Here, by using a mouse model of inflammation induced by lipopolysaccharide (LPS) injection, we investigated the effect of LPS-induced neuroinflammation on the synaptic and non-synaptic plasticity in basolateral amygdala (BLA) projection neurons (PNs) and their contribution to the LPS-induced anxiety- and depressive-like behavior. The results showed that LPS treatment led to the activation of microglia and production of proinflammatory cytokines in the BLA. Furthermore, LPS treatment increased excitatory but not inhibitory synaptic transmission due to the enhanced presynaptic glutamate release, thus leading to the shift of excitatory/inhibitory balance towards excitatory. In addition, the intrinsic neuronal excitability of BLA PNs was also increased by LPS treatment through the loss of expression and function of small-conductance, calcium-activated potassium channel. Chronic fluoxetine pretreatment significantly prevented these neurophysiological changes induced by LPS, and alleviated anxiety and depressive-like behavior, indicating that LPS-induced neuronal dysregulation of BLA PNs may contribute to the development of psychiatry disorders. Collectively, these findings provide evidence that dysregulation of synaptic and non-synaptic transmission in the BLA PNs may account for neuroinflammation-induced anxiety- and depressive-like behavior.