Eosinophil-associated microinflammation in the gastroduodenal tract contributes to gastric hypersensitivity in a rat model of early-life adversity

• Published in: American journal of physiology: Gastrointestinal and liver physiology 2021-02, Vol.320 (2), p.G206-G216
• Main Authors: Duan, Shaoqi, Kondo, Takashi, Miwa, Hiroto, Yang, Yanjing, Wang, Shenglan, Kanda, Hirosato, Kogure, Yoko, Imamura, Nobuko, Fujimura, Tadahiro, Kono, Tomoaki, Fukushima, Masashi, Tozawa, Katsuyuki, Tomita, Toshihiko, Oshima, Tadayuki, Fukui, Hirokazu, Yamamoto, Satoshi, Noguchi, Koichi, Dai, Yi
• Format: Article
• Language: English
• Subjects: Animals; Animals, Newborn; Duodenum - pathology; Eosinophils; Gastric Mucosa - innervation; Gastric Mucosa - pathology; Gastritis; Hypersensitivity; Inflammation - pathology; Maternal Deprivation; Pressure; Rats; Rats, Sprague-Dawley; Stomach - pathology; Stress, Physiological
• ISSN: 0193-1857; 1522-1547
• DOI: 10.1152/ajpgi.00313.2020

Gastric hypersensitivity is a major pathophysiological feature of functional dyspepsia (FD). Recent clinical studies have shown that a large number of patients with FD present with gastroduodenal microinflammation, which may be involved in the pathophysiology of FD. However, no animal model reflecting this clinical characteristic has been established. The underlying mechanism between microinflammation and FD remains unknown. In this study, using a maternal separation (MS)-induced FD model, we aimed to reproduce the gastroduodenal microinflammation and reveal the interaction between gastroduodenal microinflammation and gastric hypersensitivity. The MS model was established by separating newborn Sprague-Dawley rats for 2 h a day from to . At 7-8 wk of age, electromyography was used to determine the visceromotor response to gastric distention (GD) and immunohistochemistry was performed to detect distension-associated neuronal activation as well as immunohistological changes. Our results demonstrated that MS-induced FD rats underwent gastric hypersensitivity with GD at 60 and 80 mmHg, which are related to increased p-ERK1/2 expression in the dorsal horn of T9-T10 spinal cords. Eosinophils, but not mast cells, were significantly increased in the gastroduodenal tract, and the coexpression rate of CD11b and major basic protein significantly increased in MS rats. Treatment with dexamethasone reversed gastric hypersensitivity in MS-induced FD rats by inhibiting eosinophil infiltration. These findings indicated that neonatal MS stress induces eosinophil-associated gastroduodenal microinflammation and gastric hypersensitivity in adulthood in rats. Microinflammation contributes to gastric hypersensitivity; therefore, anti-inflammatory therapy may be effective in treating patients with FD with gastroduodenal microinflammation. We showed for the first time that neonatal MS stress-induced FD rats undergo gastroduodenal eosinophil-associated microinflammation in adulthood. Suppression of microinflammation attenuated gastric hypersensitivity in MS rats. These findings established a functional link between microinflammation and gastric hypersensitivity, which may provide a potential clue for the clinical treatment of FD.