Nanoprecipitation preparation of low temperature-sensitive magnetoliposomes

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2021-02, Vol.198, p.111453-111453, Article 111453
• Main Authors: Cheung, Calvin C.L., Monaco, Ilaria, Kostevšek, Nina, Franchini, Mauro Comes, Al-Jamal, Wafa T.
• Format: Article
• Language: English
• Subjects: Low temperature-sensitive liposomes; Lysolipid; Magnetoliposomes; Nanoprecipitation
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2020.111453

[Display omitted] •Prepared hydrophobic magnetic nanoparticles-containing low temperature-sensitive liposomes using nanoprecipitation.•Developed ethanol-THF binary solvent that solubilized nanoparticle-lipids mixture, enabling magnetoliposomes self-assembly.•Confirmed drug loading capability and thermosensitivity of the magnetoliposomes prepared using nanoprecipitation. Lysolipid-containing thermosensitive liposomes (LTSL) have gained attention for triggered release of chemotherapeutics. Superparamagnetic iron oxide nanoparticles (SPION) offers multimodal imaging and hyperthermia therapy opportunities as a promising theranostic agent. Combining LTSL with SPION may further enhance their performance and functionality of LTSL. However, a major challenge in clinical translation of nanomedicine is the poor scalability and complexity of their preparation process. Exploiting the nature of self-assembly, nanoprecipitation is a simple and scalable technique for preparing liposomes. Herein, we developed a novel SPION-incorporated lysolipid-containing thermosensitive liposome (mLTSL10) formulation using nanoprecipitation. The formulation and processing parameters were carefully designed to ensure high reproducibility and stability of mLTSL10. The effect of solvent, aqueous-to-organic volume ratio, SPION concentration on the mLTSL10 size and dispersity was investigated. mLTSL10 were successfully prepared with a small size (∼100 nm), phase transition temperature at around 42 °C, and high doxorubicin encapsulation efficiency. Indifferent from blank LTSL, we demonstrated that mLTSL10 combining the functionality of both LTSL and SPION can be successfully prepared using a scalable nanoprecipitation approach.