Insulin binding to the analytical antibody sandwich pair OXI‐005 and HUI‐018: Epitope mapping and binding properties

The insulin epitopes for two monoclonal antibodies (mAbs), OXI‐005 and HUI‐018, commonly used in combination for insulin concentration determination in sandwich assays, were determined using X‐ray crystallography. The crystal structure of the HUI‐018 Fab in complex with human insulin (HI) was determ...

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Bibliographic Details
Published in:Protein science 2021-02, Vol.30 (2), p.485-496
Main Authors: Johansson, Eva, Wu, Xiaoai, Yu, Bingke, Yang, Zhiru, Cao, Zheng, Wiberg, Charlotte, Jeppesen, Claus B., Poulsen, Fritz
Format: Article
Language:English
Subjects:
analytical antibody
Binding
Chains
Channeling
Crystal structure
Crystallography
Crystals
Epitope mapping
Fab
HUI‐018
Immunoassay
Insulin
Monoclonal antibodies
OXI‐005
Residues
sandwich assay
Surface plasmon resonance
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