Regenerative medicine for the hepatobiliary system: A review

• Published in: Journal of hepato-biliary-pancreatic sciences 2021-11, Vol.28 (11), p.913-930
• Main Authors: Huang, Yu, Miyamoto, Daisuke, Hidaka, Masaaki, Adachi, Tomohiko, Gu, Wei‐Li, Eguchi, Susumu
• Format: Article
• Language: English
• Subjects: Animal research; hepatocyte; liver; Liver diseases; Medicine; Regenerative medicine; Tissue engineering; transplantation
• ISSN: 1868-6974; 1868-6982
• DOI: 10.1002/jhbp.882

Liver transplantation, the only proven treatment for end‐stage liver disease and acute liver failure, is hampered by the scarcity of donors. Regenerative medicine provides an alternative therapeutic approach. Tremendous efforts dedicated to liver regenerative medicine include the delivery of transplantable cells, microtissues, and bioengineered whole livers via tissue engineering and the maintenance of partial liver function via extracorporeal support. This brief review summarizes the current status of regenerative medicine for the hepatobiliary system. For liver regenerative medicine, the focus is on strategies for expansion of transplantable hepatocytes, generation of hepatocyte‐like cells, and therapeutic potential of engineered tissues in liver disease models. For biliary regenerative medicine, the discussion concentrates on the methods for generation of cholangiocyte‐like cells and strategies in the treatment of biliary disease. Significant advances have been made in large‐scale and long‐term expansion of liver cells. The development of tissue engineering and stem cell induction technology holds great promise for the future treatment of hepatobiliary diseases. The application of regenerative medicine in liver still lacks extensive animal experiments. Therefore, a large number of preclinical studies are necessary to provide sufficient evidence for their therapeutic effectiveness. Much remains to be done for the treatment of hepatobiliary diseases with regenerative medicine. Highlight Huang and colleagues summarized the current status of regenerative medicine research on the liver and bile ducts, covering stem cell culture techniques, small molecule cocktail medium, and tissue engineering. Although there are still many challenges, the development of these technologies holds great promise for the future treatment of hepatobiliary diseases.