Regulation of autophagy by controlling Erk1/2 and mTOR for platelet-derived growth factor-BB-mediated vascular smooth muscle cell phenotype shift

• Published in: Life sciences (1973) 2021-02, Vol.267, p.118978-118978, Article 118978
• Main Authors: Han, Joo-Hui, Park, Hyun-Soo, Lee, Do-Hyung, Jo, Jun-Hwan, Heo, Kyung-Sun, Myung, Chang-Seon
• Format: Article
• Language: English
• Subjects: Actin; Animals; Antigens; Aorta; Apoptosis; Autophagy; Autophagy - physiology; Becaplermin - genetics; Becaplermin - metabolism; Becaplermin - pharmacology; Biomedical materials; Calcium-Binding Proteins; Calponin; Calponins; Cell migration; Cell Movement - drug effects; Cell Proliferation - drug effects; Cells, Cultured; Erk1/2; Extracellular signal-regulated kinase; Genotype & phenotype; Growth factors; Kinases; MAP Kinase Signaling System - physiology; Microfilament Proteins; Mitogen-Activated Protein Kinase 3 - metabolism; mTOR; Muscle, Smooth, Vascular - metabolism; Muscles; Myocytes, Smooth Muscle - metabolism; Myosin; Myosins; Osteopontin; PDGF-BB; Phagocytosis; Phenotype; Phenotype shift; Phenotypes; Platelet-derived growth factor; Platelet-derived growth factor BB; Proliferating cell nuclear antigen; Proliferating Cell Nuclear Antigen - metabolism; Proto-Oncogene Proteins c-sis - metabolism; Rapamycin; Rats; Signal transduction; Signal Transduction - drug effects; Signaling; Smooth muscle; TOR protein; TOR Serine-Threonine Kinases - metabolism; VSMC
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2020.118978

Vascular smooth muscle cell (VSMC) phenotype shift is involved in the pathophysiology of vascular injury or platelet-derived growth factor (PDGF)-induced abnormal proliferation and migration of VSMCs. We aimed to investigate the underlying mechanism involved in PDGF-mediated signaling pathways and autophagy regulation followed by VSMC phenotype shift. The proliferation, migration and apoptosis of cultured rat aortic VSMCs were measured, and cells undergoing phenotype shift and autophagy were examined. Specific inhibitors for target proteins in signaling pathways were applied to clarify their roles in regulating cell functions. PDGF-BB stimulation initiated autophagy activation and synthetic phenotype transition by decreasing α-smooth muscle-actin (SMA), calponin and myosin heavy chain (MHC) and increasing osteopontin (OPN) expression. However, U0126, a potent extracellular signal-regulated kinase 1/2 (Erk1/2) inhibitor, decreased PDGF-BB-induced LC3 expression, while rapamycin, an inhibitor of the mammalian target of rapamycin (mTOR), increased it. Furthermore, U0126 decreased the expresseion of autophagy-related genes (Atgs) such as beclin-1, Atg7, Atg5, and Atg12-Atg5 complex, indicating that Erk1/2 is a regulator of PDGF-BB-induced VSMC autophagy. Regardless of autophagy inhibition by U0126 or activation by rapamycin, the PDGF-BB-induced decrease in SMA, calponin and MHC and increase in OPN expression were inhibited. Furthermore, PDGF-BB-stimulated VSMC proliferation, migration and proliferating cell nuclear antigen (PCNA) expression were inhibited by U0126 and rapamycin. These findings suggest that PDGF-BB-induced autophagy is strongly regulated by Erk1/2, an mTOR-independent pathway, and any approach for targeting autophagy modulation is a potential therapeutic strategy for addressing abnormal VSMC proliferation and migration. [Display omitted]