Biofabrication of muscle fibers enhanced with plant viral nanoparticles using surface chaotic flows

Multiple human tissues exhibit fibrous nature. Therefore, the fabrication of hydrogel filaments for tissue engineering is a trending topic. Current tissue models are made of materials that often require further enhancement for appropriate cell attachment, proliferation and differentiation. Here we p...

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Bibliographic Details
Published in:Biofabrication 2021-07, Vol.13 (3), p.35015
Main Authors: Frías-Sánchez, Ada I, Quevedo-Moreno, Diego A, Samandari, Mohamadmahdi, Tavares Negrete, Jorge Alfonso, Sánchez-Rodríguez, Víctor Hugo, González-Gamboa, Ivonne, Ponz, Fernando, Alvarez, Mario Moisés, Trujillo de Santiago, Grissel
Format: Article
Language:English
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Summary:Multiple human tissues exhibit fibrous nature. Therefore, the fabrication of hydrogel filaments for tissue engineering is a trending topic. Current tissue models are made of materials that often require further enhancement for appropriate cell attachment, proliferation and differentiation. Here we present a simple strategy, based on the use surface chaotic flows amenable of mathematical modeling, to fabricate continuous, long and thin filaments of gelatin methacryloyl (GelMA). The fabrication of these filaments is achieved by chaotic advection in a finely controlled and miniaturized version of the journal bearing (JB) system. A drop of GelMA pregel was injected on a higher-density viscous fluid (glycerin) and a chaotic flow is applied through an iterative process. The hydrogel drop is exponentially deformed and elongated to generate a fiber, which was then polymerized under UV-light exposure. Computational fluid dynamic (CFD) simulations are conducted to determine the characteristics of the flow and design the experimental conditions for fabrication of the fibers. GelMA fibers were effectively used as scaffolds for C2C12 myoblast cells. Experimental results demonstrate an accurate accordance with CFD simulations for the predicted length of the fibers. Plant-based viral nanoparticles (i.e., Turnip mosaic virus; TuMV) were then integrated to the hydrogel fibers as a secondary nano-scaffold for cells for enhanced muscle tissue engineering. The addition of TuMV significantly increased the metabolic activity of the cell-seeded fibers (p*
ISSN:1758-5082
1758-5090
DOI:10.1088/1758-5090/abd9d7