Effect of sample type and the use of high or low fishmeal diets on bacterial communities in the gastrointestinal tract of Penaeus monodon

• Published in: Applied microbiology and biotechnology 2021-02, Vol.105 (3), p.1301-1313
• Main Authors: Noble, T. H., Truong, H. H., Rombenso, A., Simon, C. J., Wade, N. M., Wynne, J. W.
• Format: Article
• Language: English
• Subjects: Analysis; Animal Feed - analysis; Animals; Aquaculture; Bacteria; Bacteria - genetics; Bacteroidetes; Biological specimens; Biomedical and Life Sciences; Biotechnology; Contamination; Diet; Dietary supplements; Environmental Biotechnology; Firmicutes; Fish meal; Gastrointestinal system; Gastrointestinal Tract; Gene sequencing; Intestine; Life Sciences; Microbial Genetics and Genomics; Microbiology; Microbiota; Microbiota (Symbiotic organisms); Nutrient deficiency; Pellets; Penaeidae; Penaeus monodon; Physiological aspects; Polysaccharides; RNA, Ribosomal, 16S - genetics; rRNA 16S; Stomach
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-020-11052-6

In shrimp aquaculture, manufactured diets that include various supplements and alternative fishmeal ingredients are increasingly being used and their effect on the gastrointestinal (GI) microbiota studied. However, dietary effects on different shrimp GI samples are not known. We investigated how a high (HFM) or low (LFM) fishmeal diet affects bacterial communities from different sample types collected from Penaeus monodon gastrointestinal tract. Bacterial communities of the stomach, intestine tissue and intestine digesta were assessed using 16s rRNA gene sequencing. The feed pellets were also assessed as a potential source of bacteria in the GI tract. Results showed substantial differences in bacterial communities between the two diets as well as between the different sample types. Within the shrimp GI samples, stomach and digesta communities were most impacted by diet, while the community observed in the intestine tissue was less affected. Proteobacteria , Firmicutes and Bacteroidetes were the main phyla observed in shrimp samples, with enrichment of Bacteroidetes and Firmicutes in the LFM fed shrimp. The feed pellets were dominated by Firmicutes and were largely dissimilar to the shrimp samples. Several key taxa were shared however between the feed pellets and shrimp GI samples, particularly in the LFM fed shrimp, indicating the pellets may be a significant source of bacteria observed in shrimp GI samples. In summary, both diet and sample type influenced the bacterial communities characterised from the shrimp GI tract. Thus, it is important to consider the sample type collected from the GI tract when investigating dietary impacts on gut bacterial communities in shrimp. Key points • Shrimp gastrointestinal communities are influenced by diet and sample type. • The low fishmeal diet enriched bacteria that aid in polysaccharide metabolism. • Feed pellets can be a source of bacteria-detected gastrointestinal tract of shrimp.