Mineralized Human Amniotic Membrane as a Biomimetic Scaffold for Hard Tissue Engineering Applications

The human amniotic membrane (HAM) has been viewed as a potential regenerative material for a wide variety of injured tissues because of its collagen-rich content. High degradability of HAM limits its wide practical application in bone tissue engineering. In this study, the natural matrix of the dece...

Full description

Saved in:
Bibliographic Details
Published in:ACS biomaterials science & engineering 2020-11, Vol.6 (11), p.6285-6298
Main Authors: Sabouri, Leila, Farzin, Ali, Kabiri, Azadeh, Milan, Peiman Brouki, Farahbakhsh, Mojtaba, Mehdizadehkashi, Abolfazl, Kajbafzadeh, Abdolmohammad, Samadikuchaksaraei, Ali, Yousefbeyk, Fatemeh, Azami, Mahmoud, Moghtadaei, Mehdi
Format: Article
Language:English
Subjects:
Amnion
Animals
Biomimetics
Bone Regeneration
Cell Differentiation
Humans
Rats
Tissue Engineering
Tissue Engineering and Regenerative Medicine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The human amniotic membrane (HAM) has been viewed as a potential regenerative material for a wide variety of injured tissues because of its collagen-rich content. High degradability of HAM limits its wide practical application in bone tissue engineering. In this study, the natural matrix of the decellularized amniotic membrane was developed by the double diffusion method. The results confirmed a reduction of the amniotic membrane’s degradability because of the deposition of calcium and phosphate ions during the double diffusion process. Real-time PCR results showed a high expression of osteogenesis-related genes from adipose-derived mesenchymal stem cells (ADMSCs) cultured on the surface of the developed mineralized amniotic membrane (MAM). Further in vivo experiments were conducted using an MAM preseeded with ADMSCs and a critical-size rat calvarial defect model. Histopathological results confirmed that the MAM + cell sample has excellent potential in bone regeneration.
ISSN:2373-9878
2373-9878
DOI:10.1021/acsbiomaterials.0c00881