In-situ generation of large numbers of genetic combinations for metabolic reprogramming via CRISPR-guided base editing

Reprogramming complex cellular metabolism requires simultaneous regulation of multigene expression. Ex-situ cloning-based methods are commonly used, but the target gene number and combinatorial library size are severely limited by cloning and transformation efficiencies. In-situ methods such as mult...

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Bibliographic Details
Published in:	Nature communications 2021-01, Vol.12 (1), p.678-12, Article 678
Main Authors:	Wang, Yu, Cheng, Haijiao, Liu, Yang, Liu, Ye, Wen, Xiao, Zhang, Kun, Ni, Xiaomeng, Gao, Ning, Fan, Liwen, Zhang, Zhihui, Liu, Jiao, Chen, Jiuzhou, Wang, Lixian, Guo, Yanmei, Zheng, Ping, Wang, Meng, Sun, Jibin, Ma, Yanhe
Format:	Article
Language:	English
Subjects:	38/23 38/47 42/35 49/31 5' Untranslated Regions 631/1647/2234 631/326/2522 631/326/4041/3196 631/61/318 Bacillus subtilis - genetics Bacillus subtilis - metabolism Bacterial Proteins - genetics Bacterial Proteins - metabolism Binding sites Biosynthesis Catabolism Cloning Combinatorial analysis Corynebacterium glutamicum - genetics Corynebacterium glutamicum - metabolism CRISPR CRISPR-Cas Systems - genetics Deoxyribonucleic acid DNA DNA, Bacterial - genetics Escherichia coli - genetics Gene Editing - methods Gene expression Genes Genes, Bacterial - genetics Genetic diversity Genetic transformation Genetically engineered microorganisms Genome editing Genomes Glycerol Glycerol - metabolism Humanities and Social Sciences Industrial Microbiology - methods Libraries Lycopene Lycopene - metabolism Metabolic Engineering - methods Metabolic Networks and Pathways - genetics Microorganisms multidisciplinary Multigene Family - genetics Optimal yield Science Science (multidisciplinary) Transformation, Bacterial Transformations Xylose - metabolism
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