Immobilization of Serratia plymuthica by ionic gelation and cross-linking with transglutaminase for the conversion of sucrose into isomaltulose

Isomaltulose is an alternative sugar obtained from sucrose using some bacteria producing glycosyltransferase. This work aimed to optimize conditions for the immobilization of Serratia plymuthica through ionic gelation and cross-linking by transglutaminase using the sequential experimental strategy f...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2021-06, Vol.44 (6), p.1109-1118
Main Authors: Carvalho, Priscila Hoffmann, Kawaguti, Haroldo Yukio, de Souza, Weysser Felipe Cândido, Sato, Hélia Harumi
Format: Article
Language:English
Subjects:
Alginates
Alginic acid
Bacteria
Biotechnology
Calcium alginate
Calcium chloride
Chemistry
Chemistry and Materials Science
Conversion
Crosslinking
Environmental Engineering/Biotechnology
Food Science
Gelatin
Gelation
Glycosyltransferase
Immobilization
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Research Paper
Serratia plymuthica
Sucrose
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Summary:Isomaltulose is an alternative sugar obtained from sucrose using some bacteria producing glycosyltransferase. This work aimed to optimize conditions for the immobilization of Serratia plymuthica through ionic gelation and cross-linking by transglutaminase using the sequential experimental strategy for the conversion of sucrose into isomaltulose. The effect of five variables (concentrations of cell mass, alginate, gelatin, transglutaminase, and calcium chloride) was studied, as well as the interactions between them on the matrix composition for the S. plymuthica immobilization. Three experimental designs were used to optimize the concentrations of each variable to obtain higher concentration of isomaltulose. A high conversion of sucrose into isomaltulose (71.04%) was obtained by the cells immobilized in a matrix composed of alginate (1.7%), CaCl 2 (0.25 mol/L), gelatin (0.5%), transglutaminase (3.5%) and cell mass (33.5%). As a result, the transglutaminase application as a cross-linking agent improved the immobilization of Serratia plymuthica cells and the conversion of sucrose into isomaltulose.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-021-02513-x