Identification of MaWRKY40 and MaDLO1 as Effective Marker Genes for Tracking the Salicylic Acid-Mediated Immune Response in Bananas

Bananas are among the world's most important cash and staple crops but are threatened by various devastating pathogens. The phytohormone salicylic acid (SA) plays a key role in the regulation of plant immune response. Tracking the expression of SA-responsive marker genes under pathogen infectio...

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Bibliographic Details
Published in:Phytopathology 2021-10, Vol.111 (10), p.1800-1810
Main Authors: Tzean, Yuh, Hou, Bo-Han, Tsao, Shu-Ming, Chen, Ho-Ming, Cheng, An-Po, Chen, Elena Gamboa, Chou, Wei-Yi, Chao, Chih-Ping, Shen, Wei-Chiang, Chen, Chyi-Chuann, Lee, Ming-Chi, Ashraf, Iqra, Yeh, Hsin-Hung
Format: Article
Language:English
Subjects:
Crops, Agricultural
Immunity
Musa - genetics
Plant Diseases
Salicylic Acid
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Summary:Bananas are among the world's most important cash and staple crops but are threatened by various devastating pathogens. The phytohormone salicylic acid (SA) plays a key role in the regulation of plant immune response. Tracking the expression of SA-responsive marker genes under pathogen infection is important in pathogenesis elucidation. However, the common SA-responsive marker genes are not consistently induced in different banana cultivars or different organs. Here, we conducted transcriptome analysis for SA response of a banana cultivar, 'Pei-Chiao' (Cavendish, AAA genome), and identified three genes, , , and ( ) that are robustly induced upon SA treatment in both the leaves and roots. Consistent induction of these three genes by SA treatment was also detected in both the leaves and roots of bananas belonging to different genome types such as 'Tai-Chiao No. 7' (Cavendish, AAA genome), 'Pisang Awak' (ABB genome), and 'Lady Finger' (AA genome). Furthermore, the biotrophic pathogen cucumber mosaic virus elicited the expression of and in infected leaves of susceptible cultivars. The hemibiotrophic fungal pathogen f. sp. tropical race 4 (TR4) also consistently induced the expression of and in the infected roots of the f. sp. TR4-resistant cultivar. These results indicate that and can be used as reliable SA-responsive marker genes for the study of plant immunity in banana. Revealing SA-responsive marker genes provides a stepping stone for further studies in banana resistance to pathogens.
ISSN:0031-949X
1943-7684
DOI:10.1094/PHYTO-01-21-0017-R