Can dried blood spots be used to accurately measure vitamin D metabolites?

•Vitamin D metabolites were accurately quantified from DBS samples using LC-MS/MS.•The assay discriminated the C-3 epimer from standard vitamin D metabolites.•Haematocrit remains a challenge when using DBS to quantify plasma metabolites. Where conventional blood sampling is challenging, dried blood...

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Bibliographic Details
Published in:Clinica chimica acta 2021-07, Vol.518, p.70-77
Main Authors: Binks, Michael J., Bleakley, Amy S., Rathnayake, Geetha, Pizzutto, Susan, Chang, Anne B., McWhinney, Brett, Ungerer, Jacobus
Format: Article
Language:English
Subjects:
25-hydroxyvitamin D
3-epi-25-hydroxyvitamin D
Dried blood spot
LC/MS/MS
Vitamin D
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Summary:•Vitamin D metabolites were accurately quantified from DBS samples using LC-MS/MS.•The assay discriminated the C-3 epimer from standard vitamin D metabolites.•Haematocrit remains a challenge when using DBS to quantify plasma metabolites. Where conventional blood sampling is challenging, dried blood spots (DBS) provide a practical sample alternative for measuring vitamin D levels. Our study aimed to develop and evaluate a clinical pathology service-based assay suitable for measuring vitamin D in batches of DBS samples collected remote to the testing site. A high throughput liquid chromatography-tandem mass spectrometry (LC-MS/MS) method with derivatisation was developed to measure 25-hydroxyvitamin D metabolites (25OHD3, 25OHD2 and 3-epi-25OHD3) in DBS samples. The assay was validated using paired DBS and plasma samples from 37 healthy adults. The assay reproducibly (
ISSN:0009-8981
1873-3492
DOI:10.1016/j.cca.2021.03.003