Geographic origin of Explanatum explanatum (Creplin, 1847) Fukui, 1929 detected from domestic water buffaloes in Sri Lanka

• Published in: Infection, genetics and evolution genetics and evolution, 2021-07, Vol.91, p.104806-104806, Article 104806
• Main Authors: Thang, Tran Nhat, Sivakumar, Thillaiampalam, Kothalawala, Hemal, Silva, Seekkuge Susil Priyantha, Yokoyama, Naoaki, Ichikawa-Seki, Madoka
• Format: Article
• Language: English
• Subjects: Animal Distribution; Animals; Buffaloes; Explanatum explanatum; ITS2; Nad1; Paramphistomatidae - classification; Sri Lanka; Trematode Infections - parasitology; Trematode Infections - veterinary; Water buffaloes
• ISSN: 1567-1348; 1567-7257
• DOI: 10.1016/j.meegid.2021.104806

The adult stage of Explanatum explanatum has economic importance in the production of ruminants, especially water buffaloes. This species has been widely reported in the Indian sub-continent. Recently, molecular analyses to reveal the dispersal route of this species were performed in Bangladesh, Nepal, and India. In the present study, we focused on E. explanatum distributed in Sri Lanka. A total of 52 flukes were collected from water buffaloes in Sri Lanka and identified as E. explanatum based on the internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA. Analysis of the mitochondrial NADH dehydrogenase subunit 1 (nad1) gene from DNA samples detected 18 haplotypes, and five of them were identical to those from the Indian E. explanatum. The pairwise fixation index value indicated that the Sri Lankan population had a comparatively closer relationship with the Indian population than with the Bangladeshi or Nepalese populations. The Sri Lankan population showed significantly lower genetic variability than the Indian population, suggesting that the Indian population was the ancestor of the Sri Lankan population. The movement of host ruminants, including water buffaloes, was probably involved in the introduction of the fluke into Sri Lanka. The results of our study provide useful information for elucidating the geographic origin of E. explanatum distributed in the Indian subcontinent. •Amphistomes of buffaloes in Sri Lanka were molecularly identified as E. explanatum.•Sri Lankan E. explanatum had lower π value than that of the Indian population.•A close genetic relationship was found between Sri Lankan and Indian isolates.•E. explanatum might have been introduced into Sri Lanka from India.