Interval aerobic training improves bioenergetics state and mitochondrial dynamics of different brain regions in restraint stressed rats

• Published in: Molecular biology reports 2021-03, Vol.48 (3), p.2071-2082
• Main Authors: Khodagholi, Fariba, Zareh Shahamati, Shima, Maleki Chamgordani, Mona, Mousavi, Maryam Alsadat, Moslemi, Mehdi, Salehpour, Mojtaba, Rafiei, Shahrbanoo, Foolad, Forough
• Format: Article
• Language: English
• Subjects: Animal Anatomy; Animal Biochemistry; Animals; Antioxidants; Antioxidants - metabolism; Bioenergetics; Biomedical and Life Sciences; Biosynthesis; Brain; Brain - enzymology; Brain - metabolism; Brain - pathology; Citric Acid Cycle; Energy Metabolism; Enzymatic activity; Exercise; Fumarase; Glutathione; Histology; Immobilization; Life Sciences; Malate dehydrogenase; Male; Mitochondria; Mitochondria - metabolism; Mitochondrial Dynamics; Mitochondrial Proteins - metabolism; Morphology; Neurodegeneration; Original Article; Parkin protein; Physical Conditioning, Animal; Physical training; PTEN protein; Pyruvate Dehydrogenase Complex - metabolism; Rats; Rats, Wistar; Restraint, Physical; Stress, Psychological - metabolism; Superoxide dismutase; Tricarboxylic acid cycle
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-021-06177-4

Evidence has validated the prophylactic effects of exercising on different aspects of health. On the opposite side, immobilization may lead to various destructive effects causing neurodegeneration. Here, we investigated the association between exercising and mitochondrial quality for preventing the destructive effects of restraint stress in different rat brain regions. Twenty-four male Wistar rats, were randomized into four groups (n = 6), exercise, stress, exercise + stress, and control. The exercise procedure consisted of running on a rodent treadmill for 8 weeks, and rats in the stress group were immobilized for 6 h. Rats were then euthanized by decapitation and tricarboxylic acid (TCA) cycle enzyme activity, antioxidant levels, and mitochondrial biogenesis factors were assessed in the frontal, hippocampus, parietal and temporal regions using spectrophotometer and western blot technique. Based on our results, increased activity of TCA cycle enzymes in the exercised and exercise-stressed groups was detected, except for malate dehydrogenase which was decreased in exercise-stressed group, and fumarase that did not change. Furthermore, the level of antioxidant agents (superoxide dismutase and reduced glutathione), mitochondrial biogenesis factors (peroxisome proliferator-activated receptor gamma coactivator 1-alpha and mitochondrial transcription factor A), and dynamics markers (Mitofusin 2, dynamic related protein 1, PTEN induced putative kinase-1, and parkin) increased in both mentioned groups. Interestingly our results also revealed that the majority of the mitochondrial factors increased in the frontal and parietal lobes, which may be in relation with the location of motor and sensory areas. Exercise can be used as a prophylactic approach against bioenergetics and mitochondrial dysfunction.