Tackling the Selectivity Dilemma of Benzopyrylium–Coumarin Dyes in Fluorescence Sensing of HClO and SO2

Benzopyrylium–coumarin fluorescent probes for sensing hypochlorous acid (HClO) or sulfur dioxide (SO2) are unable to distinguish between HClO and SO2 because the two compounds can react with the 4-position of benzopyrylium–coumarin dyes through the nucleophilic attack. In the current work, we introd...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2021-03, Vol.93 (12), p.5194-5200
Main Authors: Han, Jinliang, Yang, Sheng, Wang, Benhua, Song, Xiangzhi
Format: Article
Language:English
Subjects:
Chemistry
Coumarin
Dyes
Fluorescence
Fluorescent indicators
Hypochlorous acid
Response time
Selectivity
Sulfur
Sulfur dioxide
Zebrafish
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Benzopyrylium–coumarin fluorescent probes for sensing hypochlorous acid (HClO) or sulfur dioxide (SO2) are unable to distinguish between HClO and SO2 because the two compounds can react with the 4-position of benzopyrylium–coumarin dyes through the nucleophilic attack. In the current work, we introduced a phenoxazine moiety to the benzopyrylium–coumarin dye to synthesize a new fluorescent probe PBC1, which can dually sense HClO and SO2 and generate distinct fluorescence signals with rapid response time and high sensitivity and selectivity. Moreover, probe PBC1 was also successfully utilized to detect intracellular HClO and SO2 in HeLa cells and zebrafish.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.0c05266