Proteomics reveals the potential mechanism of Mrps35 controlling Listeria monocytogenes intracellular proliferation in macrophages

Macrophages are sentinels in the organism which can resist and destroy various bacteria through direct phagocytosis. Here, we reported that expression level of mitochondrial ribosomal protein S35 (Mrps35) continued to decrease over infection time after Listeria monocytogenes (L. monocytogenes) infec...

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Published in:Proteomics (Weinheim) 2021-05, Vol.21 (10), p.e2000262-n/a
Main Authors: Yuan, Jiangbei, Li, Zhangfu, Li, Fang, Lin, Zewei, Yao, Siyu, Zhou, Hang, Liu, Wenhu, Yu, Haili, Liu, Yang, Liu, Fang, Li, Fei, Ran, Haiying, Zhang, Junying, Huang, Yi, Fu, Qihuan, Wang, Liting, Liu, Jikui
Format: Article
Language:English
Subjects:
Infections
Intracellular
label‐free quantitative mass spectrometry
Listeria
Listeria monocytogenes
Listeria monocytogenes infection
Macrophages
Mass spectrometry
Mass spectroscopy
Mitochondria
Mrps35
Phagocytosis
Proteins
Proteomics
Rpl22l1
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Summary:Macrophages are sentinels in the organism which can resist and destroy various bacteria through direct phagocytosis. Here, we reported that expression level of mitochondrial ribosomal protein S35 (Mrps35) continued to decrease over infection time after Listeria monocytogenes (L. monocytogenes) infected macrophages. Our results indicated that knockdown Mrps35 increased the load of L. monocytogenes in macrophages. This result supported that Mrps35 played the crucial roles in L. monocytogenes infection. Moreover, we performed the comprehensive proteomics to analyze the differentially expressed protein of wild type and Mrps35 Knockdown Raw264.7 cells by L. monocytogenes infection over 6 h. Based on the results of mass spectrometry, we presented a wide variety of hypotheses about the mechanism of Mrps35 controlling the L. monocytogenes intracellular proliferation. Among them, experiments confirmed that Mrps35 and 60S ribosomal protein L22‐like 1 (Rpl22l1) were a functional correlation or potentially a compensatory mechanism during L. monocytogenes infection. This study provided new insights into understanding that L. monocytogenes infection changed the basic synthesis or metabolism‐related proteins of host cells.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.202000262