High-Fat Diet Drives an Aggressive Pancreatic Cancer Phenotype

• Published in: The Journal of surgical research 2021-08, Vol.264, p.163-172
• Main Authors: Garcia, Denise I., Hurst, Katie E., Bradshaw, Alexandra, Janakiraman, Harinarayanan, Wang, Cindy, Camp, E. Ramsay
• Format: Article
• Language: English
• Subjects: Adenocarcinoma - etiology; Adenocarcinoma - pathology; Animals; Cell Line, Tumor - transplantation; Culture Media - metabolism; Diet, High-Fat - adverse effects; Disease Models, Animal; Epithelial-Mesenchymal Transition; High-fat diet; Humans; Metabolic syndrome; Metabolic Syndrome - complications; Metabolic Syndrome - pathology; Mice; Obesity; Oleic Acid - metabolism; Pancreas - cytology; Pancreas - pathology; Pancreatic adenocarcinoma (PDAC); Pancreatic Neoplasms - etiology; Pancreatic Neoplasms - pathology
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1016/j.jss.2020.10.007

Emerging evidence indicates associations between high-fat diet (HFD), metabolic syndrome (MetS), and increased risk of pancreatic cancer. However, individual components of an HFD that increase cancer risk have not been isolated. In addition, a specific pattern of cytokine elevation by which MetS drives pancreatic tumor progression is not well described. We hypothesized that oleic acid (OA), a major component of HFD, would augment pancreatic neoplastic processes. An orthotopic pancreatic cancer model with Panc02 cells was used to compare the effect of low-fat diet to OA-based HFD on cancer progression. Tumors were quantitated, analyzed by immunohistochemistry. In addition, serum cytokine levels were quantitated. Proliferation, migration assays, and expression of epithelial-to-mesenchymal transition factors were evaluated on Panc02 and MiaPaCa-2 pancreatic cancer cells cultured in high concentrations of OA. HFD tumor-bearing mice (n = 8) had an 18% weight increase (P