Attenuation of the extracellular matrix increases the number of synapses but suppresses synaptic plasticity through upregulation of SK channels

• Published in: Cell calcium (Edinburgh) 2021-06, Vol.96, p.102406-102406, Article 102406
• Main Authors: Dembitskaya, Yulia, Gavrilov, Nikolay, Kraev, Igor, Doronin, Maxim, Tang, Yong, Li, Li, Semyanov, Alexey
• Format: Article
• Language: English
• Subjects: Dendritic spine; Extracellular matrix; LTP; SK channel
• ISSN: 0143-4160; 1532-1991
• DOI: 10.1016/j.ceca.2021.102406

[Display omitted] •ECM attenuation triggers the appearance of new synapses in the hippocampus.•GABAergic signalling remains unaffected.•Excitability of pyramidal neurons and LTP are reduced by SK channels.•Blockade of SK channels reveals enhanced LTP.•LTP enhancement is mediated by the ROCK pathway. The effect of brain extracellular matrix (ECM) on synaptic plasticity remains controversial. Here, we show that targeted enzymatic attenuation with chondroitinase ABC (ChABC) of ECM triggers the appearance of new glutamatergic synapses on hippocampal pyramidal neurons, thereby increasing the amplitude of field EPSPs while decreasing both the mean miniature EPSC amplitude and AMPA/NMDA ratio. Although the increased proportion of ‘unpotentiated’ synapses caused by ECM attenuation should promote long-term potentiation (LTP), surprisingly, LTP was suppressed. The upregulation of small conductance Ca2+-activated K+ (SK) channels decreased the excitability of pyramidal neurons, thereby suppressing LTP. A blockade of SK channels restored cell excitability and enhanced LTP; this enhancement was abolished by a blockade of Rho-associated protein kinase (ROCK), which is involved in the maturation of dendritic spines. Thus, targeting ECM elicits the appearance of new synapses, which can have potential applications in regenerative medicine. However, this process is compensated for by a reduction in postsynaptic neuron excitability, preventing network overexcitation at the expense of synaptic plasticity.