Phosphate Transport by Communities of Arbuscular Mycorrhizal Fungi in Intact Soil Cores

• Published in: The New phytologist 2001-01, Vol.149 (1), p.95-103
• Main Authors: Jakobsen, I., Gazey, C., Abbott, L. K.
• Format: Article
• Language: English
• Subjects: 32P; Agricultural soils; Agrology; Agronomy. Soil science and plant productions; arbuscular mycorrhizas; benomyl; Biological and medical sciences; Economic plant physiology; Fundamental and applied biological sciences. Psychology; Fungi; Glomus invermaium; Hyphae; hyphal P transport; Parasitism and symbiosis; phosphate; Plant physiology and development; Plant roots; Plants; root‐free soil compartment; Soil fungi; Soil inoculation; Soil plant interactions; Soil treatment; Symbiosis; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...); Trifolium subterraneum; undisturbed soil cores
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1046/j.1469-8137.2001.00006.x

Variation in phosphate uptake capacity is reported here for natural communities of arbuscular mycorrhizal fungi associated with annual pasture plants. Tests were made of methodology for quantifying phosphate uptake by hyphae associated with clover in soil cores from pastures containing different morpho-types of the fungi. This provided a direct measure of the phosphate uptake capacity of hyphae from 32 P-labelled soil in a root-free mesh bag inserted into the centre of intact soil cores. Bicarbonate-extractable phosphorus in the soils ranged from very deficient to close to adequate for plant growth. Uptake of 32 P was related to an estimate of the length of hyphae formed in four of the five soils, but not to either the length or the proportion of roots colonized. In the fifth soil type, phosphate uptake by hyphae was negligible. Phosphate uptake by natural communities of arbuscular mycorrhizal fungi in intact soil cores can be assessed directly, and is shown to be highly variable. The experimental approach could be applied widely for field investigations of phosphate uptake by hyphal networks.