Comparative evaluation of proliferative potential and replicative senescence associated changes in mesenchymal stem cells derived from dental pulp and umbilical cord

Mesenchymal stem cells (MSC) have been widely studied for tissue regeneration and cell-based therapy. MSC can be isolated from different body tissues while several biological waste sources like dental pulp, umbilical cord, cord derived blood, amniotic fluid or urine have also emerged as potential so...

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Bibliographic Details
Published in:Cell and tissue banking 2022-03, Vol.23 (1), p.157-170
Main Authors: Das, Monalisa, Das, Ankita, Barui, Ananya, Paul, Ranjan Rashmi
Format: Article
Language:English
Subjects:
Amniotic fluid
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell culture
Cell Differentiation
Cell Proliferation
Cells, Cultured
Cellular Senescence
Dental Pulp
Life Sciences
Mesenchymal Stem Cells
Reactive oxygen species
Regenerative medicine
Senescence
Stem Cells
Transplant Surgery
Umbilical Cord
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Summary:Mesenchymal stem cells (MSC) have been widely studied for tissue regeneration and cell-based therapy. MSC can be isolated from different body tissues while several biological waste sources like dental pulp, umbilical cord, cord derived blood, amniotic fluid or urine have also emerged as potential sources of MSCs. Specifically, isolation of MSCs from such non-conventional sources show promising outcomes due to the non-invasiveness of the extraction process and high proliferation capacity of the isolated MSC. However, these stem cells also exhibit the limitation of replicative senescence in long-term culture condition. Inter-cellular reactive oxygen species is an important contributor for inducing cellular senescence under long-term culture conditions. For translational application, it becomes imperative to compare the stem cells isolated from these sources for their senescence and proliferative properties. In this study, MSC were extracted from two different sources of biological waste materials—dental pulp and umbilical cord, and compared for their proliferation capacity and replicative senescence at different passage numbers (i.e. P2 and P6). Intracellular ROS production was significantly ( p  
ISSN:1389-9333
1573-6814
DOI:10.1007/s10561-021-09926-8