Preliminary evaluation of inhibitory activity of medicinal mushroom extracts against pathogenic bacteria and spoilage yeasts

A bacterial contamination in processed foods has been a continuing challenge for the food industry. Many chemical preservatives are showing signs of inefficiencies as microorganisms build resistance. Therefore, research into novel antimicrobial compounds is critical in improving food safety. The eth...

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Bibliographic Details
Published in:Food science & technology 2021-06, Vol.145, p.111200, Article 111200
Main Authors: Chu, Hyun Sik S., Boyer, Renee, Williams, Robert, O'Keefe, Sean F.
Format: Article
Language:English
Subjects:
absorbance
Agaricus subrufescens
Antimicrobial
bacterial contamination
Bioactive compounds
disk diffusion antimicrobial test
Escherichia coli O157
ethanol
food industry
food safety
Ganoderma lucidum
Grifola frondosa
Inonotus obliquus
Lentinula edodes
Listeria monocytogenes
medicinal fungi
Medicinal mushroom
Pathogens
Phellinus linteus
Saccharomyces cerevisiae
Salmonella Typhimurium
spoilage
spoilage yeast
Yeasts
Zygosaccharomyces bailii
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Summary:A bacterial contamination in processed foods has been a continuing challenge for the food industry. Many chemical preservatives are showing signs of inefficiencies as microorganisms build resistance. Therefore, research into novel antimicrobial compounds is critical in improving food safety. The ethanolic extracts of medicinal mushrooms (MM; Agaricus blazei Murrill, Ganoderma lucidum, Grifola frondosa, Inonotus obliquus, Lentinula edodes, and Phellinus linteus) were tested for antimicrobial activities against the common foodborne pathogens (Escherichia coli O157:H7, Salmonella enterica serovar Typhimurium, and Listeria monocytogenes) and food spoilage yeast (Saccharomyces cerevisiae, Zygosaccharomyces bailii, and Zygosaccharomyces bailii/bisporus). Inhibitory activities were measured using a disk diffusion assay (DD). Additional analysis for L. monocytogenes and Z. bailii were carried out using Optical density measurements (OD). HPLC was used to fractionate MM extracts (6–8 fractions per MM). The results from DD and OD showed that most MMs displayed statistically significant inhibition compared to the ethanol control. Stronger inhibition was observed for yeasts than bacteria. Also, the antimicrobial activities were fraction dependent. Further work is required to identify the bioactive compounds. •Six medicinal mushrooms were evaluated for antimicrobial and properties.•Higher antimicrobial activities found against yeasts compared to bacteria.•Compounds with antimicrobial activity were fractionated by HPLC.
ISSN:0023-6438
1096-1127
DOI:10.1016/j.lwt.2021.111200