A panel of eight autophagy-related long non-coding RNAs is a good predictive parameter for clear cell renal cell carcinoma

Clear-cell renal cell carcinoma (ccRCC) carries a variable prognosis. Prognostic biomarkers can stratify patients according to risk, and can provide crucial information for clinical decision-making. We screened for an autophagy-related long non-coding lncRNA (lncRNA) signature to improve postoperati...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2021-03, Vol.113 (2), p.740-754
Main Authors: Gui, Cheng-Peng, Cao, Jia-Zheng, Tan, Lei, Huang, Yong, Tang, Yi-Ming, Li, Peng-Ju, Chen, Yu-Hang, Lu, Jun, Yao, Hao-Hua, Chen, Zhen-Hua, Pan, Yi-Hui, Ye, Yun-Lin, Qin, Zi-Ke, Chen, Wei, Wei, Jin-Huan, Luo, Jun-Hang
Format: Article
Language:English
Subjects:
Autophagic flux
Autophagy
Autophagy - genetics
biomarkers
Biomarkers, Tumor - genetics
Biomarkers, Tumor - metabolism
Carcinoma, Renal Cell - genetics
Carcinoma, Renal Cell - metabolism
Carcinoma, Renal Cell - pathology
decision making
genome
genomics
Humans
Kidney Neoplasms - genetics
Kidney Neoplasms - metabolism
Kidney Neoplasms - pathology
Long non-coding RNA
prognosis
Prognostic signature
renal cell carcinoma
Renal clear cell carcinoma
risk
risk assessment
RNA, Long Noncoding - genetics
RNA, Long Noncoding - metabolism
TEM
therapeutics
transmission electron microscopy
Western blot
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Summary:Clear-cell renal cell carcinoma (ccRCC) carries a variable prognosis. Prognostic biomarkers can stratify patients according to risk, and can provide crucial information for clinical decision-making. We screened for an autophagy-related long non-coding lncRNA (lncRNA) signature to improve postoperative risk stratification in The Cancer Genome Atlas (TCGA) database. We confirmed this model in ICGC and SYSU cohorts as a significant and independent prognostic signature. Western blotting, autophagic-flux assay and transmission electron microscopy were used to verify that regulation of expression of 8 lncRNAs related to autophagy affected changes in autophagic flow in vitro. Our data suggest that 8-lncRNA signature related to autophagy is a promising prognostic tool in predicting the survival of patients with ccRCC. Combination of this signature with clinical and pathologic parameters could aid accurate risk assessment to guide clinical management, and this 8-lncRNAs signature related to autophagy may serve as a therapeutic target.
ISSN:0888-7543
1089-8646
DOI:10.1016/j.ygeno.2021.01.016