Implementation of a Sample Pooling Strategy for the Direct Detection of SARS-CoV-2 by Real-Time Polymerase Chain Reaction During the COVID-19 Pandemic

Abstract Objectives To report our institutional experience in devising and implementing a pooling protocol and process for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reverse transcription polymerase chain reaction (RT-PCR) testing over a 3-month period in the fall of 2020. Methods...

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Bibliographic Details
Published in:American journal of clinical pathology 2021-06, Vol.156 (1), p.15-23
Main Authors: Chan, Clarence W, Kwon, Seunghyug, Matushek, Scott M, Ciaglia, Carol, Bethel, Cindy, Beavis, Kathleen G
Format: Article
Language:English
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Summary:Abstract Objectives To report our institutional experience in devising and implementing a pooling protocol and process for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reverse transcription polymerase chain reaction (RT-PCR) testing over a 3-month period in the fall of 2020. Methods The widespread testing implemented in the United States for detecting SARS-CoV-2 infection in response to the coronavirus disease 2019 pandemic has led to a significant shortage of testing supplies and therefore has become a major impediment to the public health response. To date, several institutions have implemented sample pooling, but publications documenting these experiences are sparse. Nasal and nasopharyngeal samples collected from low-positivity (
ISSN:0002-9173
1943-7722
DOI:10.1093/ajcp/aqab035