High-Throughput and Continuous Chaotic Bioprinting of Spatially Controlled Bacterial Microcosms

• Published in: ACS biomaterials science & engineering 2021-06, Vol.7 (6), p.2408-2419
• Main Authors: Ceballos-González, Carlos Fernando, Bolívar-Monsalve, Edna Johana, Quevedo-Moreno, Diego Alonso, Lam-Aguilar, Li Lu, Borrayo-Montaño, Karen Ixchel, Yee-de León, Juan F, Zhang, Yu Shrike, Alvarez, Mario Moisés, Trujillo-de Santiago, Grissel
• Format: Article
• Language: English
• Subjects: Bio-interactions and Biocompatibility
• ISSN: 2373-9878; 2373-9878
• DOI: 10.1021/acsbiomaterials.0c01646

Microorganisms do not work alone but instead function as collaborative microsocieties. The spatial distribution of different bacterial strains (micro-biogeography) in a shared volumetric space and their degree of intimacy greatly influences their societal behavior. Current microbiological techniques are commonly focused on the culture of well-mixed bacterial communities and fail to reproduce the micro-biogeography of polybacterial societies. Here, we bioprinted fine-scale bacterial microcosms using chaotic flows induced by a printhead containing a static mixer. This straightforward approach (i.e., continuous chaotic bacterial bioprinting) enables the fabrication of hydrogel constructs with intercalated layers of bacterial strains. These multilayered constructs are used to analyze how the spatial distributions of bacteria affect their social behavior. For example, we show that bacteria within these biological microsystems engage in either cooperation or competition, depending on the degree of shared interface. The extent of inhibition in predator–prey scenarios (i.e., probiotic–pathogen bacteria) increases when bacteria are in greater intimacy. Furthermore, two Escherichia coli strains exhibit competitive behavior in well-mixed microenvironments, whereas stable coexistence prevails for longer times in spatially structured communities. We anticipate that chaotic bioprinting will contribute to the development of a greater complexity of polybacterial microsystems, tissue-microbiota models, and biomanufactured materials.