Regional gene therapy for bone healing using a 3D printed scaffold in a rat femoral defect model

At the present time there are no consistently satisfactory treatment options for some challenging bone loss scenarios. We have previously reported on the properties of a novel 3D‐printed hydroxyapatite‐composite material in a pilot study, which demonstrated osteoconductive properties but was not tes...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part A 2021-11, Vol.109 (11), p.2346-2356
Main Authors: Kang, H Paco, Ihn, Hansel, Robertson, Djani M, Chen, Xiao, Sugiyama, Osamu, Tang, Amy, Hollis, Roger, Skorka, Tautis, Longjohn, Donald, Oakes, Daniel, Shah, Ramille, Kohn, Donald, Jakus, Adam E, Lieberman, Jay R
Format: Article
Language:English
Subjects:
3D‐printing
Animals
Biomechanics
Biomedical materials
Bone growth
Bone healing
Bone loss
Bone marrow
Bone Morphogenetic Protein 2 - biosynthesis
Bone Morphogenetic Protein 2 - genetics
Bone Regeneration - drug effects
Bone Regeneration - genetics
bone repair
Composite materials
Femur
Femur - injuries
Femur - metabolism
Gene therapy
Genetic Therapy
Healing
Hydroxyapatite
Male
Osteoconduction
Osteogenesis
Pilot Projects
Printing, Three-Dimensional
Rats
Rats, Inbred Lew
Scaffolds
stem cell
Three dimensional composites
Three dimensional printing
Tissue Scaffolds - chemistry
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Summary:At the present time there are no consistently satisfactory treatment options for some challenging bone loss scenarios. We have previously reported on the properties of a novel 3D‐printed hydroxyapatite‐composite material in a pilot study, which demonstrated osteoconductive properties but was not tested in a rigorous, clinically relevant model. We therefore utilized a rat critical‐sized femoral defect model with a scaffold designed to match the dimensions of the bone defect. The scaffolds were implanted in the bone defect after being loaded with cultured rat bone marrow cells (rBMC) transduced with a lentiviral vector carrying the cDNA for BMP‐2. This experimental group was compared against 3 negative and positive control groups. The experimental group and positive control group loaded with rhBMP‐2 demonstrated statistically equivalent radiographic and histologic healing of the defect site (p > 0.9), and significantly superior to all three negative control groups (p 
ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.37217