Targeted analysis of eicosanoids derived from cytochrome P450 pathway by high‐resolution multiple‐reaction monitoring mass spectrometry

Cytochrome P450 (CYP450) pathway is one of the critical enzymatic via eicosanoid biosynthesis. Nevertheless, their metabolites are far less explored. This pathway plays a crucial role in converting arachidonic acid to hydroxyeicosatetraenoic (HETEs), epoxyeicosatrienoic (EETs), dihydroxyeicosatetrae...

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Bibliographic Details
Published in:Journal of mass spectrometry. 2021-07, Vol.56 (7), p.e4769-n/a
Main Authors: Acunha, Tanize, Nardini, Viviani, Peti, Ana Paula Ferranti, Prado, Morgana Kelly Borges, Moraes, Luiz Alberto Beraldo, Faccioli, Lúcia Helena
Format: Article
Language:English
Subjects:
Acids
Arachidonic acid
Biological properties
Biological samples
Biosynthesis
Chromatography, Liquid
Cytochrome
Cytochrome P-450 Enzyme System
Cytochrome P450
Cytochromes
Cytochromes P450
Eicosanoids
Feasibility studies
LC–MS/MS
Lipidomics
Liquid chromatography
Mass Spectrometry
Mass spectroscopy
Metabolites
Monitoring
MRM‐HR
Resolution
Specificity
targeted lipidomics
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Summary:Cytochrome P450 (CYP450) pathway is one of the critical enzymatic via eicosanoid biosynthesis. Nevertheless, their metabolites are far less explored. This pathway plays a crucial role in converting arachidonic acid to hydroxyeicosatetraenoic (HETEs), epoxyeicosatrienoic (EETs), dihydroxyeicosatetraenoic acids (DiHETEs), and dihydroxyeicosatrienoic acids (DiHETrEs), which mediate several physiological and pathological functions. However, CYP450‐derived eicosanoids are structurally complex, making those analyses a challenge in lipidomics studies. Herein, a high‐resolution multiple‐reaction monitoring (MRMHR) method has been proposed as a powerful tool for the simultaneous analysis of CYP450‐eicosanoids on different biological samples. The developed liquid chromatography (LC)‐MRMHR method was partially validated according to the Food and Drug Administration (FDA) criteria, demonstrating adequate specificity, linearity, precision, and accuracy. Besides, several biological samples were analyzed to guarantee the feasibility of the method. The proposed strategy may improve the understanding of CYP450‐derived eicosanoids in biological systems, which could be fundamental to reveal new aspects of those in physiologic and pathologic conditions.
ISSN:1076-5174
1096-9888
DOI:10.1002/jms.4769