miR-33-3p Regulates PC12 Cell Proliferation and Differentiation In Vitro by Targeting Slc29a1

MicroRNA-33-3p (miR-33-3p) has been widely investigated for its roles in lipid metabolism and mitochondrial function; however, there are few studies on miR-33-3p in the context of neurological diseases. In this study, we investigated the functional role of miR-33-3p in rat pheochromocytoma PC12 cell...

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Bibliographic Details
Published in:Neurochemical research 2021-09, Vol.46 (9), p.2403-2414
Main Authors: Shan, Bo-Quan, Li, Wen, He, Hui, Zhao, He-Yan, Tian, Mei-Ling, Cheng, Xiang, Qin, Jian-Bing, Jin, Guo-Hua
Format: Article
Language:English
Subjects:
Acetyltransferase
Apoptosis
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell differentiation
Cell growth
Cell proliferation
Choline
Choline O-acetyltransferase
Cholinergic nerves
Cholinergics
Differentiation
Enzyme-linked immunosorbent assay
Flow cytometry
Immunofluorescence
Lipid metabolism
Lipids
miRNA
Mitochondria
Nervous system
Neurochemistry
Neurological diseases
Neurology
Neurosciences
Original Paper
Pheochromocytoma cells
Therapeutic targets
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Summary:MicroRNA-33-3p (miR-33-3p) has been widely investigated for its roles in lipid metabolism and mitochondrial function; however, there are few studies on miR-33-3p in the context of neurological diseases. In this study, we investigated the functional role of miR-33-3p in rat pheochromocytoma PC12 cells. A miR-33-3p mimic was transduced into PC12 cells, and its effects on proliferation, apoptosis, and differentiation were studied using the MTS assay, EdU labeling, flow cytometry, qRT-PCR, western blot, ELISA, and immunofluorescence. We found that miR-33-3p significantly suppressed PC12 cell proliferation, but had no effect on apoptosis. Furthermore, miR-33-3p promoted the differentiation of PC12 cells into Tuj1-positive and choline acetyltransferase-positive neuron-like cells. Mechanistically, miR-33-3p repressed the expression of Slc29a1 in PC12 cells. Importantly, knocking down Slc29a1 in PC12 cells inhibited proliferation and induced differentiation into neuron-like cells. In conclusion, this study showed that miR-33-3p regulated Slc29a1, which in turn controlled the proliferation and differentiation of PC12 cells. Thus, we hypothesize that the miR-33-3p/Slc29a1 axis could be a promising therapeutic target for recovering neurons and the cholinergic nervous system.
ISSN:0364-3190
1573-6903
DOI:10.1007/s11064-021-03377-z