Methylobrevis albus sp. nov., isolated from freshwater lake sediment

• Published in: Archives of microbiology 2021-09, Vol.203 (7), p.4549-4556
• Main Authors: Zou, Qi-Hang, Feng, Xi, Wang, Tao-Jie, Du, Zong-Jun
• Format: Article
• Language: English
• Subjects: Alphaproteobacteria - classification; Alphaproteobacteria - genetics; Archives & records; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cell Biology; China; Chromatography; Deoxyribonucleic acid; DNA; Ecology; Fatty acids; Fatty Acids - analysis; Flagella; Freshwater lakes; Gene sequencing; Genomes; Geologic Sediments - microbiology; Gram-negative bacilli; Lake sediments; Lakes; Lecithin; Life Sciences; Lipids; Methylobrevis; Microbial Ecology; Microbiology; Microscopy; Morphology; Motility; New species; Nucleotide sequence; Original Paper; Phosphatidylcholine; Phosphatidylethanolamine; Phosphatidylglycerol; Phospholipids - analysis; Phylogenetics; Phylogeny; Pleomorphomonadaceae; Quinones; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sediments; Sodium chloride; Species Specificity; Taxonomy
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-021-02442-z

An aerobic, Gram-stain-negative, rod-shaped bacterium with flagellum, designated L22 T , was isolated from sediment of Hulun Lake, Inner Mongolia, China. The organism was found to grow optimally at 30° C in a medium containing 0–0.75% (w/v) NaCl at pH 7.5. The major fatty acid identified was summed feature 8 (C 18:1 ω 7 c ). The dominant polar lipids were phosphomonoester, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The main respiratory quinone was Q-10. The draft genome sequence of strain L22 T consisted of 4354,788 bp. The G + C content of genomic DNA was 69.8 mol %. The 16S rRNA gene sequences indicated that strain L22 T was affiliated with the genus Methylobrevis within the family Pleomorphomonadaceae , being most closely related to Methylobrevis pamukkalensis JCM 30229 T with 95.9% 16S rRNA gene sequences similarity. The AAI, ANI and dDDH values between strain L22 T and M. pamukkalensis JCM 30229 T were 72.5%, 80.7% and 22.7%. Based on taxonomic results in this study, we proposed that strain L22 T a novel species in the genus Methylobrevis of the family Pleomorphomonadaceae , for which the name Methylobrevis albus sp. nov. is proposed. The type strain is L22 T (=KCTC 72858 T =MCCC 1H00432 T ).