Untargeted Phylogenetic Group III of Multi-drug-Resistant Bacillus cereus Isolated Using Fraser Medium from Retail Chickens in Ho Chi Minh City

The prevalence of food-borne bacteria in developing countries is less well understood than in developed countries. The ISO11290-1 isolation method is commonly used to study Listeria contamination in chicken; however, all isolates are identified as untargeted Bacillus cereus . This study aimed to det...

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Bibliographic Details
Published in:Current microbiology 2021-08, Vol.78 (8), p.3115-3123
Main Authors: Nakayama, Tatsuya, Yamaguchi, Takahiro, Jinnai, Michio, Yamamoto, Shiori, Li, Hien Thi, Ngo, Phong Thanh, Tran, Doan Nguyen Minh, Nguyen, Oanh Thi Hoang, Hoang, Phuong Hoai, Do Nguyen, Phuc, Van Dang, Chinh, Kumeda, Yuko, Hase, Atsushi
Format: Article
Language:English
Subjects:
Ampicillin
Antibiotics
Bacillus cereus
Bacteria
Biomedical and Life Sciences
Biotechnology
Cereulide
Chickens
Ciprofloxacin
Developed countries
Developing countries
Drug resistance
Enterotoxins
Food
Food contamination
Food safety
Foodborne diseases
Genes
LDCs
Life Sciences
Listeria
Microbiology
Multilocus sequence typing
Phylogenetics
Phylogeny
Poultry
Strains (organisms)
Virulence
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Summary:The prevalence of food-borne bacteria in developing countries is less well understood than in developed countries. The ISO11290-1 isolation method is commonly used to study Listeria contamination in chicken; however, all isolates are identified as untargeted Bacillus cereus . This study aimed to determine the classification, antibiotic susceptibility, and virulence genes of B. cereus isolated from retail chickens in Vietnam. Bacterial isolation using the ISO11290-1 method yielded 12 strains of B. cereus from seven out of 60 chickens. For determining bacterial diversity, pan C and multilocus sequence typing (MLST) analyses were performed. PanC analysis showed that all seven strains belong to the phylogenetic group III, to which the highest risk of foodborne illnesses was associated. MLST analysis showed that most strains contained a ST205 complex; further, all strains were found to be resistant to ampicillin, ciprofloxacin, and tetracycline. Virulence genes were also investigated. ces, a cereulide-related gene, was detected in 50% of the isolated strains, followed by cyt K , nhe A , and hbl A enterotoxins in 41.7%, 16.7%, and 25% of the strains, respectively. In conclusion, B. cereus may be erroneously detected when attempting to detect Listeria in food using the ISO11290-1 method. Further study of the prevalence of B. cereus in Vietnamese food is needed to improve food safety.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-021-02562-1