Effect of Predation by Colpoda sp. in Nitrogen Fixation Rate of Two Free-Living Bacteria

• Published in: Microbial ecology 2022-05, Vol.83 (4), p.1026-1035
• Main Authors: Martínez-Reyes, Carlos Manuel, Rodríguez-Zaragoza, Salvador, Cabirol, Nathalie, Alarcón, Alejandro, Mendoza-López, Ma. Remedios
• Format: Article
• Language: English
• Subjects: Acetylene; Animals; Bacteria; Biomedical and Life Sciences; Ciliophora; Colpoda; Ecology; Ethene; Ethylene; Geoecology/Natural Processes; Growth rate; Interspecific relationships; Kinetics; Life Sciences; Microbial activity; Microbial Ecology; Microbiology; Microorganisms; Nature Conservation; Nitrogen; Nitrogen Fixation; Nitrogen-fixing bacteria; Nitrogenase; Nitrogenation; Population growth; Predation; Predators; Predatory Behavior; Prey; Prokaryotes; Protozoa; Soil Microbiology; Stenotrophomonas; Water Quality/Water Pollution
• ISSN: 0095-3628; 1432-184X
• DOI: 10.1007/s00248-021-01813-9

Biological nitrogen fixation is limited to several groups of prokaryotes, some of them reduce nitrogen as free-living nitrogen-fixing bacteria. Protozoa predation on these latter releases sequestered nitrogen that may enhance the formation of new bacterial biomass and possibly increase nitrogen fixation within soil microbial communities. We aim to evaluate the predation effect of Colpoda sp. on two nitrogen fixers: Azospirillum lipoferum and Stenotrophomonas sp. during their lag, early exponential, and exponential phases. The kinetics of bacterial population growth was determined in the predators’ presence or absence and the effect of predation on the rate of N fixation was evaluated through the reduction of acetylene to ethylene technique. Colpoda sp. showed a non-significant difference in preferences between the two species offered as prey. Consequently, the abundance of A. lipoferum and Stenotrophomonas sp. decreased significantly due to predator’s pressure and both species responded by increasing their specific growth rate. Likewise, predation promoted greater nitrogen fixation rate by CFU during the lag phase in A. lipoferum (0.20 nM/CFU with predation vs 0.09 nM/CFU without predation) and Stenotrophomonas sp. (0.22 nM/CFU vs 0.09 nM/CFU respectively). During early exponential phase (29 h), the rate diminished to 0.13 and 0.05 nM/CFU in A. lipoferum and to 0.09 nM/CFU and 0.05 nM/CFU in Stenotrophomonas sp. Finally, during the exponential phase (52 h), only A. lipoferum without predation produced 0.003 nM/CFU of ethylene. Thus, the nitrogenase activity was higher in the lag and the early exponential phases when predator activity was involved. Graphical abstract