Functional dissection of the phosphotransferase system provides insight into the prevalence of Faecalibacterium prausnitzii in the host intestinal environment

Summary Faecalibacterium prausnitzii is a dominant member of healthy human colon microbiota, regarded as a beneficial gut bacterium due to its ability to produce anti‐inflammatory substances. However, little is known about how F. prausnitzii utilizes the nutrients present in the human gut, influenci...

Full description

Saved in:
Bibliographic Details
Published in:Environmental microbiology 2021-08, Vol.23 (8), p.4726-4740
Main Authors: Kang, Deborah, Ham, Hyeong‐In, Lee, Seung‐Hwan, Cho, Yong‐Joon, Kim, Yeon‐Ran, Yoon, Chang‐Kyu, Seok, Yeong‐Jae
Format: Article
Language:English
Subjects:
Carbohydrates
Colon
Components
Digestive system
Dissection
Enzyme I
Enzymes
Faecalibacterium prausnitzii
Faecalibacterium prausnitzii - metabolism
Gastrointestinal tract
Genes
Humans
Inflammation
Intestine
Intestines
Microbiota
N-Acetylglucosamine
Nutrients
Phosphorylation
Phosphotransferase
Phosphotransferases - genetics
Phosphotransferases - metabolism
Prevalence
Regulatory proteins
Saccharides
Species
Transport
Transportation systems
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Summary Faecalibacterium prausnitzii is a dominant member of healthy human colon microbiota, regarded as a beneficial gut bacterium due to its ability to produce anti‐inflammatory substances. However, little is known about how F. prausnitzii utilizes the nutrients present in the human gut, influencing its prevalence in the host intestinal environment. The phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS) is a widely distributed and highly efficient carbohydrate transport system found in most bacterial species that catalyses the simultaneous phosphorylation and import of cognate carbohydrates; its components play physiological roles through interaction with other regulatory proteins. Here, we performed a systematic analysis of the 16 genes encoding putative PTS components (2 enzyme I, 2 HPr, and 12 enzyme II components) in F. prausnitzii A2‐165. We identified the general PTS components responsible for the PEP‐dependent phosphotransfer reaction and the sugar‐specific PTS components involved in the transport of two carbohydrates, N‐acetylglucosamine and fructose, among five enzyme II complexes. We suggest that the dissection of the functional PTS in F. prausnitzii may help to understand how this species outcompetes other bacterial species in the human intestine.
ISSN:1462-2912
1462-2920
DOI:10.1111/1462-2920.15681