Diversity and abundance of the functional genes and bacteria associated with RDX degradation at a contaminated site pre- and post-biostimulation

Bioremediation is becoming an increasingly popular approach for the remediation of sites contaminated with the explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX). Multiple lines of evidence are often needed to assess the success of such approaches, with molecular studies frequently providing im...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2021-08, Vol.105 (16-17), p.6463-6475
Main Authors: Dang, Hongyu, Cupples, Alison M.
Format: Article
Language:English
Subjects:
Abundance
Bacteria
Biodegradation
Biomarkers
Biomedical and Life Sciences
Bioremediation
Biotechnology
Chemical properties
Contamination
Deoxyribonucleic acid
DNA
Environmental aspects
Environmental Biotechnology
Explosives
Genes
Genetic aspects
Groundwater
Hexahydro-1,3,5-trinitro-1,3,5-triazine
Life Sciences
Methods
Microbial Genetics and Genomics
Microbiology
Physiological aspects
Pseudomonas fluorescens
Pseudomonas putida
RDX
Relative abundance
Species
Triazine
Water analysis
Water sampling
Water wells
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Summary:Bioremediation is becoming an increasingly popular approach for the remediation of sites contaminated with the explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX). Multiple lines of evidence are often needed to assess the success of such approaches, with molecular studies frequently providing important information on the abundance of key biodegrading species. Towards this goal, the current study utilized shotgun sequencing to determine the abundance and diversity of functional genes ( xenA , xenB , xplA , diaA , pnrB , nfsI ) and species previously associated with RDX biodegradation in groundwater before and after biostimulation at an RDX-contaminated Navy Site. For this, DNA was extracted from four and seven groundwater wells pre- and post-biostimulation, respectively. From a set of 65 previously identified RDX degraders, 31 were found within the groundwater samples, with the most abundant species being Variovorax sp. JS1663, Pseudomonas fluorescens , Pseudomonas putida , and Stenotrophomonas maltophilia . Further, 9 RDX-degrading species significantly ( p 30 RDX-degrading species were detected. • Nine RDX-degrading species increased following biostimulation. • Sequencing and high-throughput qPCR indicated that xenA and xenB were most abundant.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-021-11457-x