Circulating microRNA breast cancer biomarker detection in patient sera with surface plasmon resonance imaging biosensor

In this article, we report for the first time, the detection of circulating miRNA as a breast cancer biomarker in patient sera using surface plasmon resonance imaging biosensor. The advantage of this approach lies in the rapid, label‐free and sensitive detection. The sensor excites plasmonic resonan...

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Bibliographic Details
Published in:Journal of biophotonics 2021-11, Vol.14 (11), p.e202100153-n/a
Main Authors: Wong, Chi Lok, Loke, Sau Yeen, Lim, Hann Qian, Balasundaram, Ghayathri, Chan, Patrick, Chong, Bee Kiang, Tan, Ern Yu, Lee, Ann Siew Gek, Olivo, Malini
Format: Article
Language:English
Subjects:
Biomarkers
Biomarkers, Tumor - genetics
Biosensing Techniques
Biosensors
Breast cancer
breast cancer biomarker
Breast Neoplasms - diagnostic imaging
Breast Neoplasms - genetics
Circulating MicroRNA
circulating miRNA
clinical study
Deoxyribonucleic acid
DNA
Female
Gold
Humans
Malignancy
MicroRNAs
miRNA
Patients
Plasmonics
Resonance
Ribonucleic acid
RNA
Sensors
SPR imaging
Surface Plasmon Resonance
surface plasmon resonance biosensor
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Summary:In this article, we report for the first time, the detection of circulating miRNA as a breast cancer biomarker in patient sera using surface plasmon resonance imaging biosensor. The advantage of this approach lies in the rapid, label‐free and sensitive detection. The sensor excites plasmonic resonance on the gold sensor surface and specific DNA‐miRNA molecular bindings elucidate responses in the plasmonic resonance image. Experiments of detecting synthetic miRNA molecules (miR‐1249) were performed and the sensor resolution was found to be 63.5 nM. The sensor was further applied to screen 17 patient serum samples from National Cancer Centre Singapore and Tan Tock Seng Hospital. Sensor intensity response was found to differ by 20% between malignant and benign cases and thus forms, a potential and an important metric in distinguishing benignity and malignancy. In this work, we report for the first time the detection of circulating miRNA as a breast cancer biomarker in patient sera using surface plasmon resonance imaging biosensor. The sensor was applied to screen 17 patient serum samples from National Cancer Centre Singapore and Tan Tock Seng Hospital. The detection time was 25 mins and the sensor intensity response was found to differ by 20% between malignant and benign cases and, thus, forms a potential and important metric in distinguishing benignity and malignancy.
ISSN:1864-063X
1864-0648
DOI:10.1002/jbio.202100153