Annotation of chemotaxis gene clusters and proteins involved in chemotaxis of Bacillus subtilis strain MB378 capable of biodecolorizing different dyes

This study explores the chemotactic potential of Bacillus subtilis MB378 against industrial dyes. Initial screening with swim plate assay showed significant movement of Bacillus subtilis MB378 towards test compounds. According to quantitative capillary assay, B. subtilis MB378 exhibited high chemota...

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Bibliographic Details
Published in:Environmental science and pollution research international 2022, Vol.29 (3), p.3510-3520
Main Authors: Tahir, Uruj, Aslam, Fozia, Nawaz, Shiza, Khan, Umair Hassan, Yasmin, Azra
Format: Article
Language:English
Subjects:
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Aquatic Pollution
Atmospheric Protection/Air Quality Control/Air Pollution
Bacillus subtilis
Bacillus subtilis - genetics
Bacteria
Bacterial Proteins - genetics
Bioavailability
Biodegradation
Chemotaxis
Coloring Agents - metabolism
Detoxification
Dyes
Earth and Environmental Science
Ecotoxicology
Environment
Environmental Chemistry
Environmental Health
Environmental science
Flagella
Gene clusters
Gene sequencing
Genes
Genomes
Malachite green
Membrane Proteins - genetics
Methyl-Accepting Chemotaxis Proteins - genetics
Molecular motors
Multigene Family
Protein families
Proteins
Research Article
Waste Water Technology
Water Decolorization
Water Management
Water Pollution Control
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Summary:This study explores the chemotactic potential of Bacillus subtilis MB378 against industrial dyes. Initial screening with swim plate assay showed significant movement of Bacillus subtilis MB378 towards test compounds. According to quantitative capillary assay, B. subtilis MB378 exhibited high chemotaxis potential towards Acid Orange 52 (CI: 9.52), followed by Direct Red 28 (CI: 8.39) and Basic Green 4 (CI: 5.21) in glucose-supplemented medium. Sequencing and gene annotation results evidently showed presence of chemotaxis genes and flagellar motor proteins in Bacillus subtilis draft genome. Methyl-accepting proteins (involved in chemotaxis regulation) belonged to pfam00672, pfam00072, and pfam00015 protein families. Annotated chemotaxis machinery of MB378 comprised 8 Che genes, 5 chemoreceptor genes, associated flagellar proteins, and rotary motors. Chemotaxis genes of B. subtilis MB378 were compared with genes of closely related Bacillus strains (168, WK1, and HTA426), depicting highly conserved regions showing evolutionary relation between them. Considering results of present study, it can be speculated that test compounds triggered chemotactic genes, which made these compounds bioavailable to the bacterium. Hence, the bacterium recognized and approached these compounds and facilitated biodegradation and detoxification of these compounds. Graphical abstract
ISSN:0944-1344
1614-7499
DOI:10.1007/s11356-021-15634-3