Effect of nickel on red blood cell parameters and on serum vitamin B12, folate and homocysteine concentrations during pregnancy with and without anemia

• Published in: Journal of trace elements in medicine and biology 2021-12, Vol.68, p.126839-126839, Article 126839
• Main Authors: Pieczyńska, Joanna, Płaczkowska, Sylwia, Sozański, Rafał, Skórska, Katarzyna, Sołtysik, Martyna
• Format: Article
• Language: English
• Subjects: Anemia; Erythrocytes; Female; Folate; Folic Acid; Homocysteine; Humans; Nickel; Pregnancy; Red blood cell parameters; Vitamin B 12; Vitamin B12
• ISSN: 0946-672X; 1878-3252
• DOI: 10.1016/j.jtemb.2021.126839

•The pregnant women with the development of pregnancy had a slightly variable dietary nickel intake.•We observed a significant changes in whole blood nickel concentration and urine nickel excretion during pregnancy.•The whole blood nickel concentration was significantly higher in anemic than non- anemic pregnant women.•The whole blood nickel concentrations is associated to red blood cell parameters in non-anemic pregnant women. Research to date suggests that nickel affects not only the metabolism of vitamin B12 but also folates and thus may affect hematopoiesis processes. The aim of the study was to examine the relationship of nickel (Ni) status to red blood cell (RBC) parameters and serum vitamin B12, folate and homocysteine concentrations in the course of normal pregnancy and in pregnant women with anemia. The study included fifty-three pregnant women recruited to the study from the Lower Silesia region of Poland, 17 % of whom developed anemia. Nickel concentration was determined in urine, whole blood and food samples by atomic absorption spectrometry. At the same time as the food and urine samples were taken, blood was also collected for the determination of RBC parameters and serum vitamin B12, homocysteine and folate concentrations. The median reported Ni intake, and the urinary and whole blood nickel contents for the studied pregnant women for the first trimester were respectively – 162.46 μg/day, 3.98 μg/L and 3.32 μg/L; for the second trimester – 110.48 μg/day, 6.86 μg/L and 1.04 μg/L; and for the third trimester – 132.20 μg/day, 3.41 μg/L and 0.70 μg/L. With regard to Ni concentration in whole blood (p = 0.0204) and in urine (p = 0.0003), the differences in the values for individual trimesters were statistically significant. The whole blood Ni level was significantly higher (9.28 vs 3.62 μg/L, p = 0.0114), while the concentration of homosysteine was significantly lower (4.09 vs 5.04 μmol/L, p = 0.0165) in pregnant women with anemia compared to those without anemia. The whole blood Ni concentration was negatively correlated with almost all RBC parameters in non-anemic pregnant women. Ni status changes with the development of normal pregnancy, and in the case of anemia, an increase in Ni concentration in whole blood is observed. The demonstrated correlations between the Ni status in pregnant women and RBC parameters as well as serum vitamin B12 and folate concentrations suggest that nickel is associated with the methionine–folate cycle, iron homeost