In‐bone protein digestion followed by LC‐MS/MS peptide analysis as a new way towards the routine proteomic characterization of human maxillary and mandibular bone tissue in oral surgery

Proteomic characterization of alveolar bones in oral surgery represents an analytical challenge due to their insoluble character. The implementation of a straightforward technique could lead to the routine use of proteomics in this field. This work thus developed a simple technique for the character...

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Bibliographic Details
Published in:Electrophoresis 2021-12, Vol.42 (23), p.2552-2562
Main Authors: Hynek, Radovan, Michalus, Iva, Cejnar, Pavel, Šantrůček, Jiří, Seidlová, Sabina, Kučková, Štěpánka, Sázelová, Petra, Kašička, Václav
Format: Article
Language:English
Subjects:
Bones
Chromatography, Liquid
Digestion
Discrimination
Humans
In‐bone protein digestion
LC‐MS/MS
Mandible - metabolism
Mandibular bone
Maxilla - metabolism
Maxillary bone
Oral surgery
Oral Surgical Procedures
Peptide mapping
Peptides
Proteins
Proteins - metabolism
Proteolysis
Proteomics
Statistical analysis
Statistical methods
Surgery
Tandem Mass Spectrometry
Trypsin - metabolism
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Summary:Proteomic characterization of alveolar bones in oral surgery represents an analytical challenge due to their insoluble character. The implementation of a straightforward technique could lead to the routine use of proteomics in this field. This work thus developed a simple technique for the characterization of bone tissue for human maxillary and mandibular bones. It is based on the direct in‐bone tryptic digestion of proteins in both healthy and pathological human maxillary and mandibular bone samples. The released peptides were then identified by the LC‐MS/MS. Using this approach, a total of 1120 proteins were identified in the maxillary bone and 1151 proteins in the mandibular bone. The subsequent partial least squares–discrimination analysis (PLS‐DA) of protein data made it possible to reach 100% discrimination between the samples of healthy alveolar bones and those of the bone tissue surrounding the inflammatory focus. These results indicate that the in‐bone protein digestion followed by the LC‐MS/MS and subsequent statistical analysis can provide a deeper insight into the field of oral surgery at the molecular level. Furthermore, it could also have a diagnostic potential in the differentiation between the proteomic patterns of healthy and pathological alveolar bone tissue. Data are available via ProteomeXchange with the identifier PXD026775.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.202100211